Cell-Free Protein Production

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In recent years, cell-free protein synthesis technology has experienced a revolution. This book details latest techniques in cell-...

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Beschreibung

In recent years, cell-free protein synthesis technology has experienced a revolution. This book details latest techniques in cell-free protein production, including needed materials, step-by-step directions, lab protocols, and troubleshooting tips.
During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.
Covers cell-free expression systems from E. coli, wheat, insect, and human cells as well as a totally reconstituted system Shows how cell-free expression can be applied to high-throughput and one-by-one protein functional and structural analyses Provides rapid methods for protein engineering and the creation of new proteins Presents basic methodologies that can support constitutive approaches for understanding complex molecular systems Includes supplementary material: sn.pub/extras
Inhalt
1. Cell-Free Protein Preparation through Prokaryotic Transcription-Translation Methods Takanori Kigawa 2. PURE Technology Yoshihiro Shimizu and Takuya Ueda 3. The Cell-Free Protein Synthesis System from Wheat Germ Kazuyuki Takai and Yaeta Endo 4. A Cell-Free Protein Synthesis System from Insect Cells Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, and Eiji Ando 5. Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells Satoshi Mikami, Tominari Kobayashi, and Hiroaki Imataka 6. Analysis of Protein Functions through a Bacterial Cell-Free Protein Expression System Takanori Kigawa 7. Cell-Free Based Protein Microarray Technology Using Agarose/DNA Microplate Tatsuya Sawasaki and Yaeta Endo 8. An Efficient Approach to the Production of Vaccines Against the Malaria Parasite Takafumi Tsuboi, Satoru Takeo, Tatsuya Sawasaki, Motomi Torii, and Yaeta Endo 9. Protein Engineering Accelerated by Cell-Free Technology Takuya Kanno and Yuzuru Tozawa 10. Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds Takanori Kigawa 11. NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System Toshiyuki Kohno 12. Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination Shin-ichi Makino, Michael A. Goren, Brian G. Fox, and John L. Markley 13. Cell-Free Protein Synthesis for Structure Determination by X-Ray Crystallography Miki Watanabe, Ken-ichi Miyazono, Masaru Tanokura, Tatsuya Sawasaki, Yaeta Endo, and Ichizo Kobayashi 14. Production of Multi-Subunit Complexes on Liposome through an E. coli Cell-Free Expression System Yutetsu Kuruma,Toshiharu Suzuki, and Takuya Ueda 15. Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System Hiroyuki Hori 16. Strategies for the Cell-Free Expression of Membrane Proteins Sina Reckel, Solmaz Sobhanifar, Florian Durst, Frank Löhr, Vladimir A. Shirokov, Volker Dötsch, and Frank Bernhard 17. Production of Membrane Proteins through the Wheat-Germ Cell-Free Technology Akira Nozawa, Hideaki Nanamiya, and Yuzuru Tozawa 18. Ribosome Display with the PURE Technology Takashi Kanamori, Hiroyuki Ohashi, and Takuya Ueda 19. Incorporation of 3-azidotyrosine into Proteins through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification Takashi Yokogawa, Satoshi Ohno, and Kazuya Nishikawa 20. Synthesis of Functional Proteins within Liposomes Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, and Tetsuya Yomo