Cancer Susceptibility

Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease. In Cancer Susceptibility: Methods and Protocols, experts in the field bring together the most recent technological developments for identifying and screening cancer susceptibility genes. Divided into two clear sections, the book begins with gene identification, which updates and informs scientists working at identifying novel cancer susceptibility genes, while the second part deals with mutation screening technologies that aid scientists and clinicians working to translate this knowledge into the clinic. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Susceptibility: Methods and Protocols is a timely collection that seeks to provide researchers with the tools to predict and combat this terrible disease.

Contains convenient protocols dealing with the characterization, identification, and screening of cancer genes Lists expert tips to aid the processes Provides step-by-step, readily reproducible instruction Includes supplementary material: sn.pub/extras

Inhalt
Identifying Cancer Susceptibility Genes.- The Identification of Colon Cancer Susceptibility Genes by Using Genome-Wide Scans.- Prioritizing Candidate Genetic Modifiers of BRCA1 and BRCA2 Using a Combinatorial Analysis of Global Expression and Polymorphism Association Studies of Breast Cancer.- Microarray-Based Comparative Genomic Hybridization (Array-CGH) as a Useful Tool for Identifying Genes Involved in Glioblastoma (GB).- Multiplex Amplifiable Probe Hybridization (MAPH) Methodology as an Alternative to Comparative Genomic Hybridization (CGH).- Utilizing Saccharomyces Cerevisiae to Identify Aneuploidy and Cancer Susceptibility Genes.- Computational Identification of Cancer Susceptibility Loci.- Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes.- Screening Cancer Susceptibility Genes.- The Use of Denaturing High Performance Liquid Chromatography (DHPLC) for Mutation Scanning of Hereditary Cancer Genes.- Enhanced Mismatch Mutation Analysis: Simultaneous Detection of Point Mutations and Large Scale Rearrangements by Capillary Electrophoresis, Application to BRCA1 and BRCA2.- Economical Protocol for Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis on a Standard Capillary Electrophoresis Apparatus.- Mutational Screening of hMLH1 and hMSH2 that Confer Inherited Colorectal Cancer Susceptibility Using Denature Gradient Gel Electrophoresis (DGGE).- s-RT-MELT: A Novel Technology for Mutation Screening.- Zoom-In Array Comparative Genomic Hybridization (aCGH) to Detect Germline Rearrangements in Cancer Susceptibility Genes.- Development of a Scoring System to Screen for BRCA1/2 Mutations.- Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic Variants.- Functional Analysis of Human BRCA2 Variants Using a Mouse Embryonic Stem Cell-Based Assay.- Developing Functional Assays for BRCA1 Unclassified Variants.