Calcium Measurement Methods

Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field.

Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

Provides a comprehensive overview of calcium imaging of the neural cells Presents a detailed account of state-of the art calcium indicators including fluorescent, luminescent, and genetically modified calcium probes Outlines wide array of techniques aimed at real-time calcium imaging of cellular organelles, subcellular compartments, individual cells, and neural networks Covers the theoretical and practical background of calcium indicators, as well as calcium imaging hardware and software Comprehensive guide to conceiving, developing, and running calcium imaging of nervous system

Inhalt
Principles of the Ca2+ Homeostatic/Signalling System.- Ca2+ Recordings: Hardware and Software (From Microscopes to Cameras).- Ca2+ Imaging: Principles of Analysis and Enhancement.- Bioluminescent Ca2+ Indicators.- Monitoring Calcium Levels With Genetically Encoded Indicators.- Intracellular Calcium-Sensitive Microelectrodes.- Ca2+ Caging and Uncaging.- Ca2+ Imaging of Intracellular Organelles: Endoplasmic Reticulum.- Ca2+ Imaging of Intracellular Organelles: Mitochondria.- Ca2+ Imaging of Dendrites and Spines.- In Vivo Ca2+ Imaging of the Living Brain Using Multi-cell Bolus Loading Technique.- Ca2+ Imaging of Glia.