This second edition details the techniques involved in the biosafety assessment of probiotics to be used for human consumption. New and updated chapters guide readers through identification of antibiotic resistance and virulence genes using various databases, PCR techniques, in vitro cytotoxicity assays, cytolysins, and in vivo toxicity assays such as acute and chronic toxicity, virulence, toxigenicity and dermonecrotic effects. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.

Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential, Second Eidition aims to be comprehensive guide for researchers in the fiel d.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Detection of virulence and antibiotic resistance associated genes by PCR.- Determination of antibiotic resistance by turbidometric method.- Determination of Cytotoxicity of Probiotics by Cell Viability Assays.- Determination of cytotoxicity by lacta dehydrogenase activity.- Determination of Cytotoxicity by Calcein-Propidium Iodide Staining for live and dead cell detection.- Determination of Cytotoxicity by Edu Cell Proliferation Assay.- Detection of Biofilm Formation.- In vitro assessment of cytolysins production by hemolysis assays.- In vitro assessment of cytolysins' genes by PCR.- In vitro assessment of cytolysin proteins by ELISA.- In vitro assessment of cytolysin proteins by Western Blotting.- In vitro assessment of cytolysins' production by cytotoxicity assays.- In vitro assessment of cytolysins production by Mass Spectrometry.- In vivo assessment of cytolysins production.- Identification of Antibiotic Resistance Genes Using the Comprehensive Antibiotic Resistance Database (CARD).- Identification of virulence genes through virulence factor database (VFDB).- Determination of assessment of genes related to Biogenic Amines through Rapid Annotations using Subsystems Technology (RAST).- In vivo assessment of microbial translocation.- In vivo determination of acute oral toxicity.- In vivo determination of virulence.- In vivo determination of toxigenicity.- In vivo determination of acute and chronic toxicity.- In vivo determination of dermonecrotic properties.- In vivo assessment of genotoxicity by Mouse Spermatogonial Chromosomal Aberration Test.- In vivo assessment of genotoxicity using COMET assay.- In vitro Cyto-genotoxicity assessment of probiotics, using cytokinesis-block micronucleus (CBMN) assay in grown peripheral human lymphocytes.- In vivo assessment of genotoxicity of probiotics by Mammalian Erythrocyte Micronucleus Test.