This volume details methods and protocols of extraction, separation, characterization, and quantification of phytochemicals. Chapters cover extraction techniques, for instance, solvent extraction and supercritical fluid extraction, UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), gas chromatography (GC), and liquid chromatography (LC) coupled with mass spectrometry (MS), used to measure the concentration of bioactive compounds. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.

Authoritative and cutting-edge, Phytochemicals : Extraction, Quantification, Isolation, and Characterization aims to be comprehensive guide for ** researchers in the field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Extraction and Quantification of Polyphenols.- Extraction and Quantification of Plant Based Carotenoids.- Extraction of Fats and Quantification of Health Promoting Fatty acids.- Extraction and Quantification of Health Promoting Phytosterols.- Extraction and Quantification of Alkaloids.- Extraction and quantification of dietary fibres and health-promoting oligosaccharides.- Extraction and Quantification of Health Promoting Peptides.- Extraction of Lectin FRIL from leguminous plant Lablab purpureus.- Isolation and characterization of polyphenols.- Isolation and Characterization of Carotenoids.- Isolation and Characterization of Fatty Acids.- Isolation and Characterization of Health promoting Phytosterols.- Isolation and Characterization of Health-Promoting Alkaloids.- Isolation and characterization of health-promoting polypeptides.- Isolation and Characterization of Health Promoting Oligosaccharides.- Chromatography (HPLC-DAD) methods for analysing alkaloids from plants.- In-vitro evaluation of phytochemicals for their health-promoting potential.- LC-MS/MS and GC-MS method for the determination of phenolics in plant tissue.