This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Animal Coronaviruses: A Brief Introduction.- Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues.- Virus Neutralization Assay for Turkey Coronavirus Infection.- Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli.- Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen.- Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus.- Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses.- Real-Time RT-PCR Detection of Equine Coronavirus.- Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction.- Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus.- An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes.- A Multiplex Polymerase Chain Reaction forDifferential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus.- Real-Time Reverse Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues.- PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene.- Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis.- Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction.- Molecular Characterization of Canine Coronavirus.- Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform.- Next Generation Sequencing for Porcine Coronaviruses.