The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation.

Provides a single all-inclusive resource for the theoretical and practical aspects of sample preparations for molecular biology, proteomics, and glycomics-related studies Covers both the theoretical foundations and practical recipes for successful sample preparation processes and tasks dealing with difficult samples Systematizes most of the tribal knowledge of the field, and sheds a further light on this process flow which is, for many researchers, the most occasional reason for a bottleneck in a lab Includes supplementary material: sn.pub/extras

Autorentext

Miodrag Mickey Mii , Ph.D., Sc.D., M.T.M., C.S.W.P. is the Department Chair and Professor at the Department of Engineering Design Technology and Department of New Products Development at Cerritos College. He is also a Project Scientist at the BioMEMS Lab, Department of Mechanical and Aerospace Engineering, University of California, Irvine. He is a Visiting Professor at the Faculty of Applied EcologyFutura at Singidunum University, Belgrade, Serbia. Dr. Mii is a consultant to several life sciences, medical devices, and biotech companies. Prior to his current position at Cerritos College, Dr. Mii was the Vice President of Research and Development at MP Biomedicals LLC (Formerly ICN Biomedicals Inc), Irvine, CA, USA. Earlier in his career, he was a Senior Application Scientist at Veeco Metrology, Santa Barbara, CA, USA. Dr. Mii did his postdoctoral work as a Postdoctoral Research Fellow at Pacifi c Northwest National Laboratories, Richland, WA, USA. He is theauthor of 67 publications in leading scientific journals, with over 1,400 citations. Dr. Mii holds a Ph.D. and M.S. degrees in Chemistry from the University of Miami, Coral Gables, FL, USA. He holds an M.T.M. degree from Washington State University, WA, USA. Further, Dr. Mii holds Sc.D. and M.Sc. degrees in Physical Chemistry; a B.Sc./B.Ed. in Physics and Fundamentals of Engineering from the University of Belgrade, Belgrade, Serbia; and a B.Eng. degree in Mechanical/Naval Architecture Engineering from the Polytechnic Academy of New Belgrade, Belgrade, Serbia. He is also an FAA licensed instrument-rated commercial pilot and a PADI master scuba diver.

Inhalt

Physical and Chemical Properties of Nucleic Acids.- Sampling for Biostatistics.- Sampling of Microbiological Samples.- Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples.- Magnetic beads based nucleic acids purification for molecular biology applications.- Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications.- Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples.- High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and proteins extraction with FastPrep® Systems.- Portable Systems for Sample Lysis and Homogenization.- DNA and RNA extractions from mammalian samples.- Sample preparation for multiplex PCR assays for food and agriculture applications.- Nucleic acid sample preparation from stem cells.- Nucleic Acids Sample Preparation from Teeth/Dental Remains.- Nucleic Acids Sample Preparation for Food Traceability.- Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis.- Nucleic Acids Samples Preparation from Dairy Products and Milk.- Extraction of DNA from Plant Tissue: Review and Protocols.- DNA Extraction from Seeds.- RNA extractions from difficult to prepare and high starch content seeds.- Nucleic Acid and Protein Sample Preparation from Yeasts.- Nucleic acids purification from soil and environmental sources.- Extraction and Purification of Viral Nucleic Acids from Environmental Samples.- Preparation of nucleic acids from marine samples: applications to microbial ecology research.- Nucleic Acid Sample Preparation from Feces and Manure.- Bead-beating sample preparation for nucleic acids isolation from fecal samples.- Methods for extraction and purification of lignin and cellulose from plant tissues.- Separation and purification of glycans out of glycoproteins.- Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria.