Advanced Protocols in Oxidative Stress II

This second volume of Advanced Protocols in Oxidative Stress focuses on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data.

Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress , all included in the highly successful Methods in Molecular Biology ™ series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved.

Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.

Contains new total antioxidant capacity assays Includes colorimetric assays for hydroxyl radical and ethylene production analysis Explains the use of Laser Induced Breakdown Spectroscopy technique to analyze metals Provides oxidative hemolysis inhibition assay Examines the synthesis of alpha lipoic acid hybrids Explores the clinical use of pentaerythrityl tetranitrate as an antioxidant Delves into topics such as erythrocyte plasma membrane redox system, separation of thiols by HPLC-ECD, global index for determining oxidative balance, near patient testing, and in vivo pO2 using phosphorescence quenching Studies subjects like cellular chemiluminescence probe for redox state, 8-Isoprostane in breath condensate, oximetry and redoximetry using electron paramagnetic resonance spectroscopy, mitochondrial membrane potential, proton leak, and peroxide production Involves protocols on erythrocyte osmotic fragility as a biomarker for oxidative stress, high-throughput microplate assay for COX activity, effect of oxidative stress on insulin function, and redox imaging Delivers information on fluorescence-activated flow cytometry to measure oxidative stress Presents production and purification protocols for adenovirus vectors encoding antioxidant enzymes Offers examples of ChIP analysis for determining caveolin-1 gene transcription Includes supplementary material: sn.pub/extras

Inhalt
Reactive Oxygen and Nitrogen Techniques.- Current Status of Measuring Oxidative Stress.- pO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia.- Nitrate Reductase Activity of Mitochondrial Aldehyde Dehydrogenase (ALDH-2) as a Redox Sensor for Cardiovascular Oxidative Stress.- Identification of ROS Using Oxidized DCFDA and Flow-Cytometry.- Measurement of 8-Isoprostane in Exhaled Breath Condensate.- Electron Paramagnetic Resonance Oximetry and Redoximetry.- Measurement of Mitochondrial Membrane Potential and Proton Leak.- Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage.- Using N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro.- Structural and Functional Changes in the Insulin Molecule Produced by Oxidative Stress.- Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo.- Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa.- Lipofuscin: Detection and Quantification by Microscopic Techniques.- Antioxidant Technology and Application.- OXY-SCORE: A Global Index to Improve Evaluation of Oxidative Stress by Combining Pro- and Antioxidant Markers.- Cupric Ion Reducing Antioxidant Capacity Assay for Antioxidants in Human Serum and for Hydroxyl Radical Scavengers.- Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method.- Measuring Antioxidant Capacity Using the ORAC and TOSC Assays.- Assessing the Neuroprotective Effect of Antioxidant Food Factors by Application of Lipid-Derived Dopamine Modification Adducts.- LIBS-Based Detection of Antioxidant Elements: A New Strategy.- A Method for Evaluation of AntioxidantActivity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis.- Design and Synthesis of Antioxidant ?-Lipoic Acid Hybrids.- Characterization of the Antioxidant Properties of Pentaerithrityl Tetranitrate (PETN)-Induction of the Intrinsic Antioxidative System Heme Oxygenase-1 (HO-1).- Direct Determination of Tissue Aminothiol, Disulfide, and Thioether Levels Using HPLC-ECD with a Novel Stable Boron-Doped Diamond Working Electrode.- Activation of Erythrocyte Plasma Membrane Redox System Provides a Useful Method to Evaluate Antioxidant Potential of Plant Polyphenols.- Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus Stearothermophilus.- Separation of Phenylpropanoids and Evaluation of Their Antioxidant Activity.- Gene Expression.- Generation of Antioxidant Adenovirus Gene Transfer Vectors Encoding CuZnSOD, MnSOD, and Catalase.- HPLC Purification of Adenoviral Vectors.- Mapping of Oxidative Stress Response Elements of the Caveolin-1 Promoter.- Biostatistics.- Meta-Analysis: Drawing Conclusions When Study Results Vary.