This second volume of Advanced Protocols in Oxidative Stress focuses on novel techniques for detecting ROS/RNS, unique AOX technol...
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This second volume of Advanced Protocols in Oxidative Stress focuses on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data.
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular Biology™ series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved. Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.
Contains new total antioxidant capacity assays Includes colorimetric assays for hydroxyl radical and ethylene production analysis Explains the use of Laser Induced Breakdown Spectroscopy technique to analyze metals Provides oxidative hemolysis inhibition assay Examines the synthesis of alpha lipoic acid hybrids Explores the clinical use of pentaerythrityl tetranitrate as an antioxidant Delves into topics such as erythrocyte plasma membrane redox system, separation of thiols by HPLC-ECD, global index for determining oxidative balance, near patient testing, and in vivo pO2 using phosphorescence quenching Studies subjects like cellular chemiluminescence probe for redox state, 8-Isoprostane in breath condensate, oximetry and redoximetry using electron paramagnetic resonance spectroscopy, mitochondrial membrane potential, proton leak, and peroxide production Involves protocols on erythrocyte osmotic fragility as a biomarker for oxidative stress, high-throughput microplate assay for COX activity, effect of oxidative stress on insulin function, and redox imaging Delivers information on fluorescence-activated flow cytometry to measure oxidative stress Presents production and purification protocols for adenovirus vectors encoding antioxidant enzymes Offers examples of ChIP analysis for determining caveolin-1 gene transcription Includes supplementary material: sn.pub/extras Inhalt Part I: Reactive Oxygen and Nitrogen Techniques 1. Current Status of Measuring Oxidative Stress Beniamino Palmieri and Valeriana Sblendorio 2. PO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia Silvia Bertuglia and Marcos Intaglietta 3. Nitrate Reductase Activity of Mitochondrial Aldehyde Dehydrogenase (ALDH-2) as a Redox Sensor for Cardiovascular Oxidative Stress Andreas Daiber and Thomas Münzel 4. Identification of ROS Using Oxidized DCFDA and Flow-Cytometry Evgeniy Eruslanov and Sergei Kusmartsev 5. Measurement of 8-Isoprostane in Exhaled Breath Condensate Paolo Montuschi, Peter J. Barnes, and Giovanni Ciabattoni 6. Electron Paramagnetic Resonance Oximetry and Redoximetry Guanglong He 7. Measurement of Mitochondrial Membrane Potential and Proton Leak Gaetano Serviddio and Juan Sastre 8. Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage Bechan Sharma, Devendra K. Rai, Prashant Kumar Rai, S.I. Rizvi, and Geeta Watal 9. Using N, N, N', N'-Tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro Nenad Petrovic and Michael Murray 10. Structural and Functional Changes in the Insulin Molecule Produced by Oxidative Stress Rafael Medina-Navarro, Alberto M. Guzmán-Grenfell, Ivonne Olivares-Corichi, and Juan J. Hicks 11. Multiphoton Redox Ratio Imaging for Metabolic Monitoring in vivo Melissa Skala and Nirmala Ramanujam 12. Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa H. David Guthrie and Glenn R. Welch 13. Lipofuscin: Detection and Quantification by Microscopic Techniques Tobias Jung,Annika Höhn, and Tilman Grune Part II: Antioxidant Technology and Application 14. Oxy-Score: A Global Index to Improve Evaluation of Oxidative Stress by Combining Pro- and Antioxidant Markers Fabrizio Veglia, Viviana Cavalca, and Elena Tremoli 15. CUPRAC (Cupric Ion Reducing Antioxidant Capacity) Assay for Antioxidants in Human Serum and for Hydroxyl Radical Scavengers Resat Apak, Kubilay Güçlü, Mustafa Özyürek, Burcu Bektasoglu, and Mustafa Bener 16. Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method Kazuo Sakai, Satoko Kino, Masao Takeuchi, Tairin Ochi, Giuseppe Da Cruz, and Isao Tomita 17. Measuring Antioxidant Capacity Using the ORAC and TOSC Assays Andrew R. Garrett, Byron K. Murray, Richard A. Robison, and Kim L. O'Neill 18. Assessing the Neuroprotective Effect of Antioxidant Food Factors by Application of Lipid-Derived Dopamine Modification Adducts Xuebo Liu, Naruomi Yamada, and Toshihiko Osawa 19. LIBS-Based Detection of Antioxidant Elements: A New Strategy Geeta Watal, Bechan Sharma, Prashant Kumar Rai, Dolly Jaiswal, Devendra K. Rai, Nilesh K. Rai, and A.K. Rai 20. A Method for Evaluation of Antioxidant Activity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis Jun Takebayashi, Jianbin Chen, and Akihiro Tai 21. Design and Synthesis of Antioxidant a-Lipoic Acid Hybrids Maria Koufaki and Anastasia Detsi 22. Characterization of the Antioxidant Properties of Pentaerithrityl Tetranitrate (PETN)-Induction of the Intrinsic Antioxidative System Heme Oxygenase-1 (HO-1) Andreas Daiber and Thomas Münzel 23. Direct Determination of Tissue Aminothiol, Disulfide, and Thioether Levels Using HPLC-ECD with a Novel Stable Boron-Doped