Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology.

Complementary volumes 9 & 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

While many of the changes in recent fluorescence have been well-received, its continued growth in the world has created a challenge in trying to archive and document its use. Subsequently Chris D. Geddes has now become co-series editor of the Topics in Fluorescence Spectroscopy series. Geddes and Lakowicz have also recently launched the Reviews in Fluorescence series, which is published annually and meant to compliment the Topics in Fluorescent Spectroscopy series, with small chapters summarizing the yearly progress in fluorescence Includes supplementary material: sn.pub/extras

Autorentext
Dr Chris D. Geddes, Ph.D., Professor, has extensive experience in fluorescence spectroscopy and Plasmonics, particularly in fluorescence sensing and metal-fluorophore interactions (Metal-Enhanced Fluorescence), publishing over 200 papers and 20 books. Dr Geddes is internationally known in fluorescence for his scholarly publications and for the development of fluorescence-based plasmonics. He is the editor-in-chief of the Journal of Fluorescence and founding editor of the Who s Who in Fluorescence and Annual Reviews in Fluorescence volumes. In addition, due to the labs pioneering efforts in the fields of metallic nanoparticle-fluorophore interactions, Dr Geddes launched the Springer Journal "Plasmonics" in 2005. Dr Geddes is Director of the Institute of Fluorescence at the University of Maryland Baltimore County which focuses on the nano-bio-technological applications of fluorescence. Dr Geddes frequently chairs NIH study sections and is currently a permanent member of the NIH EBIT study section.
http://theinstituteoffluorescence.com/

Inhalt
Protein-Based Biosensors with Polarization Transduction.- GFP Sensors.- Fluorescent Saccharide Sensors.- Fluorescent Pebble Nano-Sensors and Nanoexplorers for Real-Time Intracellular and Biomedical Applications.- Aptamers as Emerging Probes for Macromolecular Sensing.- Molecular Imprinting.- Excimer Sensing.- Lifetime Based Sensors / Sensing.