The application of immobilized enzymes in medicine is the main objective of this book. The author reviews natural and synthetic carriers for enzyme immobilization, chemistry of enzyme binding, and in-vitro and in-vivo properties of immobilized enzymes. Four chapters are dedicated to clinical use of immobilized enzymes.

Inhalt

1 Introduction.- 2 Enzymes in Medicine: Advantages and Disadvantages.- 2.1 Native Enzymes in Therapy and Clinical Analysis.- 2.2 Difficulties Connected with Enzyme Application in Medicine...- 2.3 How the Immobilization can Help.- 3 Immobilization of Therapeutic Enzymes.- 3.1 General Principles of Enzyme Stabilization and Immobilization.- 3.2 The Peculiarities of Enzyme Immobilization for Medical Use. Carriers and Immobilization Methods Required.- 3.3 The Fate of Immobilized Enzymes in the Body.- 3.4 Principal Types of Immobilized Enzymes in Medicine.- 4 Therapeutic Immobilized Enzymes for Parenteral Application.- 4.1 Preparations Intended for Prolonged Circulation in the Body.- 4.1.1 Enzymes Modified with Soluble Polymers.- 4.1.1.1 Carriers and Methods of Immobilization; Properties of Immobilized Enzymes.- 4.1.1.2 Thrombolytic Therapy with Immobilized Enzymes.- 4.1.2 Artificial Cells.- 4.1.2.1 Properties and Application of Enzymes Immobilized in Semipermeable Microcapsules.- 4.1.2.2 Properties and Application of Enzymes Immobilized in Liposomes.- 4.1.2.3 Properties and Application of Enzymes Immobilized in Red Blood Cells Ghosts.- 4.2 Insoluble Immobilized Enzymes for Local Application.- 4.2.1 Slow-Release Systems and Their Classification.- 4.2.2 Polymeric Enzyme-Containing Implants and Micoparticles with Immobilized Enzymes.- 4.2.3 Enzymes for Modification of Polymeric Implants and Prosthetic Devices.- 4.3 Immobilization Principles and Drug Targeting.- 4.3.1 Aims and Tasks of Drug Targeting.- 4.3.2 Principal Systems of Drug Targeting.- 4.3.3 Direct and Indirect Conjugation of Therapeutic Enzymes with Specific Vectors.- 4.3.4 Use of Microreservoirs for Targeted Drug Transport.- 4.3.5 Enzyme Immobilization on Magnetic Carriers.- 5 Immobilized Enzymes for Extracorporeal Application.- 5.1 Immobilized Enzymes in Extracorporeal Systems.- 5.2 Dressing and Drainage Materials Containing Immobilized Enzymes; Films, Ointments and Powders with Immobilized Enzymes in the Treatment of Wounds, Ulcers and Burns.- 6 Immobilized Enzymes in Clinical Analysis.- 6.1 Enzyme Electrodes.- 6.2 Enzyme Immunoassay.- 6.3 Immobilized Bioluminiscent Systems.- 6.4 Reactors with Immobilized Enzymes; Miscellaneous.- 7 Conclusion.- 8 References.