The Lipoprotein Molecule

The amount of recent information collected about the molecular composition, structure, and function of the plasma lipoproteins, in man as well as in experimental animals, is very large. In this volume an assessment of our knowledge as it stands today has been organized within a framework of four general topics, the first about analytical data, the second about structure, the third about metab­ olism, and the last about molecular variation and pathology. Thus the analytical, theoretical, experimental, and applied aspects of the topic have been treated in conjunction with each other. The analytical data in human and nonhuman primates were obtained after ultracentrifugal or electrophoretic separation of the protein class from the native serum. Comparisons of various methods were not forgotten. The main categories of lipoproteins, namely High Density (HDL), Low Density (LDL), and Very Low Density (VLDL), are treated separately, and lipid moiety and the polypeptide moiety are carefully described. In the theoretical field of reassembly of lipoprotein particles by relipidizing delipidated polypeptides, the structural aspects of lipoprotein and the stabilizing effect of phospholipids on the poly­ peptide structure were studied. The opposite direction of the pro­ cess, namely degradation of a lipoprotein by means of lipolytic enzymes, has also been investigated and described.

Inhalt

Introductory Remarks.- The Challenge of the Lipoprotein Molecule.- Isolation and Composition of the Lipoprotein Molecule.- The Prognosis for Quantitative Lipoprotein Electrophoresis.- Solution Properties of Apolipoproteins.- Apolipoprotein Quantitation.- Isolation and Composition of Human Plasma Apolipoproteins.- The Plasma Lipoproteins in Nonhuman Primates.- Structure of the Lipoprotein Molecule.- Physical Properties, Chemical Composition and Structure of Circulating Lipoproteins.- The Physico-Chemical Properties of Lipids in Lipoproteins.- The Use of Paramagnetic Probes in the Structure Determination of High Density Serum Lipoproteins.- Reassembly of Apoproteins and Lipids.- Metabolism of the Lipoprotein Molecule.- Interconversion of Plasma Lipoproteins.- Assay of Lipoprotein Lipase in Postheparin Plasma after Suppression of the Hepatic Triglyceride Lipase with Sodium Dodecyl Sulfate.- Phospholipase A2 Interaction with Various Phospholipids. A Model for the Lipoprotein Molecule?.- Molecular Variation and Pathology.- Familial Lipoprotein Deficiency.- Familial Hypercholesterolaemia.- Biochemistry of Plasmalipoproteins in Liver Diseases.- The Role of Lipoproteins in Human Atherosclerosis (Lecture I).- The Interaction Between Hyperlipidaemia and Other Risk Factors in the Pathogenesis of Atherosclerosis (Lecture II).- Models for the Study of Tissue Deposition and Synthesis of Lipoproteins (Lecture III).- Dietary Effects on Lipoprotein Composition.- Conclusion.- Lipoprotein Molecule or Lipoprotein Particle?.