The first meeting of the NATO/CCMS Pilot Study "Dose-Response Analysis and Biologically-Based Risk assessment for Initiator and Promoter Carcinogens" was held in Rome, Italy, in the spring of 1991, and was followed by annual or bi-annual meetings held in Germany, Greece, Netherlands, Portugal, USA, up to the end of 1995; in large part supported by NATO/CCMS grants or fellowships, and organized by Pilot Study participants. The Pilot Study activity has been characterized by a higly collaborative atmosphere, which was essential for a deep and detailed analysis of a problem on which different points of view, methodological approaches and regulations exist in the various member countries. The Pilot Study was aimed at proposing a carcinogenic risk assessment procedure which is based on a detailed analysis of the relevant biological processes, and may also consent the verification of hypotheses. The specific form of theoretical and mathe­ matical models is identified by considering and using the whole set of objective data available. The multidisciplinary approach of the pilot study is reflected by the struc­ ture of this book. Each chapter is the result of the cooperation of several authors from to produce a comprehensive manual that includes different countries; its objective was both theoretical and practical information.

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1. Introduction.- 1.1. Dose-Response Assessment in Nato Countries.- 1.1.1. European Community.- 1.1.1.1. European Union.- 1.1.1.2. The Netherlands.- 1.1.1.3. United Kingdom.- 1.1.1.4. Germany.- 1.1.1.5. Denmark.- 1.1.1.6. Norway.- 1.1.1.7. Other countries.- 1.1.1.8. Concluding remarks.- 1.1.2. United States.- 1.1.2.1. Use of Dose-Response Assessment.- 1.1.2.2. Evolution of Dose-Response Assessment.- 1.1.3. Differences between the United States and European Countries.- 1.2. Future Directions in Dose-Response Assessment.- 1.3. Brief Considerations on Some Commonly Used Parameters.- 1.3.1. Variation in Carcinogenic Potency and in Parameters Adopted for Carcinogen Regulation.- 1.3.2. Toxicity Data and Carcinogenic Potencies: Correlation between Parameters Adopted for Risk Assessment.- 1.3.3. The Linearized Multistage Model and Benchmark Dose (BD) Approaches: Dose-Response Analysis May Provide a Unique Framework for Both the Carcinogenic and Noncarcinogenic Procedures.- 1.4. Structure of this Report.- 1.5. References.- 2. The Biological Basis of Cancer.- 2.1. Introduction.- 2.2. Cell Proliferation.- 2.3. Cell Proliferation and Mutation.- 2.4. Differences in Susceptibility.- 2.5. Mechanisms of Inhibition in Mutagenesis and Carcinogenesis.- 2.5.1. Introduction.- 2.5.2. Inhibition in Mutagenesis and Carcinogenesis.- 2.5.3. Extracellular Inhibition.- 2.5.4. Intracellular Inhibition.- 2.5.5. Inhibitors of Cancer Initiation.- 2.5.6. Inhibitors of Tumor Promotion and Progression.- 2.5.7. Dual Effects of Inhibitors.- 2.6. References.- 3. Sources of Data For Cancer Risk Assessment.- 3.1. Introduction.- 3.2. In Vitro and Short Term Testing.- 3.3. Trends in Animal Toxicology Testing.- 3.4. Cell Proliferation.- 3.4.1. Quantitative Methods and Data Sources.- 3.4.1.1. Direct Measurements of Cell Division.- 3.4.1.2. Serum Biomarkers of Cellular Proliferation.- 3.4.1.3. Cell kinetics of EAF.- 3.5. Sources of Toxicokinetic Data.- 3.5.1. Introduction.- 3.5.2. Model Parameters.- 3.5.2.1. Physiologic.- 3.5.2.2. Biochemical.- 3.5.3. Toxicokinetic Data.- 3.6. Inter- and Intra-Species Variability.- 3.6.1. Variability in Genetic Damage.- 3.6.2. The Parallelogram Model.- 3.7. References.- 4. Use of Biochemical and Molecular Biomarkers For Cancer Risk Assessment in Humans.- 4.1. Introduction.- 4.2. The Initiatory Complex and its Modulators.- 4.2.1. Biomarkers of Exposure.- 4.2.1.1. The External Dose.- 4.2.1.2. The Internal Dose.- 4.2.1.3. The Biologically Effective Dose.- 4.2.1.4. Interaction with Relevant Macromolecules.- 4.2.1.5. Cytogenetic Biomarkers of Early Effects.- 4.2.1.6. Discussion about the Biomarkers of Exposure.- 4.2.2. Biomarkers of Individual Susceptibility.- 4.2.2.1. Phase I Enzymes and Related Markers.- 4.2.2.2. Phase II Enzymes.- 4.2.3. DNA Repair and its Variability.- 4.2.3.1. Assessment of DNA Repair.- 4.2.3.2. Mismatch Repair, Microsatellite Instability and Mutator Phenotype.- 4.2.3.3. Other Genetic Instability Syndromes.- 4.2.3.4. Restatement of the DNA Repair Problem.- 4.2.4. Exogenous Nutritional Factors.- 4.3. The Determinants of the Clonal Expansion of the Initiated Cells.- 4.3.1. Basic Mechanisms.- 4.3.2. Cell Cycle Control Mechanisms.- 4.3.2.1. p53.- 4.3.2.2. The Rb tumour suppressor gene.- 4.3.2.3. The myc Oncogene.- 4.3.2.4. Low Molecular Weight Regulatory Proteins.- 4.3.3. Growth Factors, Growth Factor Receptors and Signal Transduction Pathways.- 4.3.3.1. Growth Factors and Receptors.- 4.3.3.2. Growth Factor Receptors.- 4.3.4. Signal Transduction Pathways.- 4.3.4.1. Transmembrane Receptors with Intrinsic TRK Activity.- 4.3.4.2. Receptors with Seven Transmembrane-spanning Domains.- 4.3.4.3. Cytoskeletal Signal Transduction Pathways.- 4.3.5. The Outcome: The Clonal Expansion of the Initiated Cells.- 4.3.5.1. Proliferation.- 4.3.5.2. Apoptosis.- 4.4. Adjuvant Determinants of the Clonal Expansion.- 4.4.1. Oxidative Damage and its Repair.- 4.4.1.1. Identification of Oxidative Damage.- 4.4.1.2. Thymine Glycol and Thymidine Glycol.- 4.4.1.3. 8-Hydroxydeoxyguanosine (8OHdG).- 4.4.1.4. ADPRT.- 4.4.1.5. Others.- 4.4.2. Intercellular Communication.- 4.4.3. Intercellular Adhesion.- 4.4.4. Cell-Surface Structures.- 4.4.5. Miscellaneous Determinants.- 4.4.5.1. Immune Status.- 4.4.5.2. Nutritional Status.- 4.5. Conclusion.- 4.6. Acknowledgments.- 4.7. References.- 5. The Multistage Model of Carcinogenesis: A Critical Review of its Use.- 5.1. Introduction.- 5.2. Historical Antecedents of the Multistage Model.- 5.3. The Armitage-Doll Multistage Model.- 5.4. Derivation, Rationale and Mathematical Form of the Model.- 5.5. The "Linearized Multistage Model".- 5.6. Time-Dependent Non-Constant Exposure Patterns: Their Influence on Multistage-Derived Risk Estimates.- 5.7. Consideration of Pharmakokinetics in Multistage Modeling.- 5.8. The Problem of Multiple Exposure: Multistage Carcinogenesis Theory and Additive and Multiplicative Models.- 5.9. Critical Review of the Model.- 5.10. Discussion.- 5.11. References.- 6. Biologically Based Models of Carcinogenesis.- 6.1. Introduction.- 6.2. A Brief History of Biologically-Based Cancer Models.- 6.3. Two-Mutation Clonal Expansion Model.- 6.4. Modes of Action of Carcinogens.- 6.5. Quantitative Formulation of the Model.- 6.5.1. The Probability of Tumor.- 6.5.1.1. Solution for Piecewise Constant Parameters.- 6.5.1.2. Identifiability of Model Parameters.- 6.6. Likelihood Construction and Estimation.- 6.7. Quantitative Analysis of Intermediate Lesions.- 6.7.1. Modeling Initiation and Promotion of EAF.- 6.7.2. Gompertz Growth.- 6.7.3. Statistical Analysis.- 6.7.4. Joint Analysis of Premalignant and Malignant Lesions.- 6.8. Toxicokinetics in Biologically Based Risk Assessment.- 6.8.1. Physiologically-based Toxicokinetic Models in Risk Assessment.- 6.8.2. Multistage Modeling.- 6.8.3. Biologically-based Risk Assessment.- 6.8.4. Model Development and Parameterization.- 6.9. Interspecies Extrapolation.- 6.9.1. Scaling Physiologic and Metabolic PBTK Model Parameters.- 6.9.2. Discussion of Allometric Scaling.- 6.9.3. Choice of a Dose Surrogate.- 6.9.4. Interspecies Extrapolation of Toxicokinetics.- 6.10. Implications for Low-Dose Extrapolation.- 6.11. References.- 7. Statistical Issues in the Application of Multistage and Biologically Based Models.- 7.1. Introduction.- 7.2. Characterization Of Models.- 7.2.1. Model Components.- 7.2.2. Model Comparison.- 7.2.3. Low Dose.- 7.3. Statistical Inference.- 7.3.1. Available Data and Parameter Estimation.- 7.3.2. Comparing Low-Dose Extrapolations from Different Models: A Simulation Study.- 7.3.3. Design of the Simulation Study.- 7.3.3.1. Data Generation.- 7.3.3.2…