This book reviews three-dimensional (3D) stem cell culture and proof of concept for organ regeneration. The chapters present studies based on developmental biology but not tissue engineering using bio-degradative scaffolds. The ultimate goal of regenerative therapy, the next generation of regenerative therapy, is to develop fully functioning bioengineered 3D organs that can replace lost or damaged organs following disease, injury, or aging. Next-generation regenerative therapy will consist of organ-replacement regenerative therapy, which aims to reproduce reciprocal epithelial and epithelial interactions and epithelial and mesenchymal interactions that occur during embryogenesis. The book then discusses the generation of several 3D functional organs and organoids such as brain, inner ear, tooth, hair, salivary glands, lacrimal glands, gastrointestinal organs, kidney, liver, and lung. This work will appeal to a wide readership such as medical scientists, developmental biologists, clinicians, and patients. The volume provides valuable information and ideas to form a next-generation field of science.

Autorentext

Takashi Tsuji

Laboratory for Organ Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan

Inhalt
Preface.- 1 Generation of various telencephalic regions from human embryonic stem cells in three dimensional culture.- 2 Self-organized cerebellar tissue from human pluripotent stem cells and its application to clinical medicine.- 3 Functional Pituitary Tissue formation Recapitulating hypothalamus and pituitary development using ES/iPS cells.- 4 Inner Ear Organoids: Recapitulating Inner Ear Development in 3D Culture.- 5 Functional Tooth Regeneration.- 6 Functional Hair Follicle Regeneration.- 7 Functional salivary gland regeneration.- 8 Functional Lacrimal Gland Regeneration.- 9 Generation of Gastrointestinal Organoids derived from Human Pluripotent Stem Cells.- 10 Early kidney specification and its recapitulation by pluripotent stem cells.- 11 Recapitulating development to generate kidney organoid cultures.- 12 Liver Regeneration using Cultured Liver Bud.- 13 Development and Bioengineering of Lung Regeneration.