Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

Klappentext

Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described here in detail. Leading experts present the required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers' addresses, helpful Internet resources, as well as specific applications, e.g. in legal medicine, microbiology and agriculture.

Inhalt

Current Protocols - Alexander Kraev. An Introduction to DNA Fingerprinting using RLFP and RAPD Techniques - R.J. Snowdon and A. Langsdorf. Pulsed-Field Gel Electrophoresis for Genomic Fingerprinting of Pathogenic Bacteria - Michael B. Coulthart, Wendy M. Johnson and Fraser Ashton. Ultrasensitive Silver Based Stains for Nucleic Acid Detection - Carl R. Merril, Karen M. Washart and Robert C. Allen. The Analysis of Microsatellites by Silver Staining of Non-denaturing Polyacrylamide Gels - Shirin Susan Joseph. Capillary Electrophoretic Separation of DNA Fragments - John M. Butler and Dennis J. Reeder. Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis - Sameer Sakallah. Electrophoresis in the Field of Forensic Science - Hildegard Haas-Rochholz. The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions - Michael Fried and Mark M. Garner. Mobility Shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis - Michael G. Harrington. Polyacrylamide Gel Electrophoresis (PAGE) of DNA/Protein Complexes - A. Kyas, W. Maeueler, J.T. Epplen. Detection of DNA Curvature using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis - David L. Wheeler