Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.

Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

Inhalt

Part I: Isolation and Culture of Endothelial Cells Chapter 1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial Cells Nancy Laurens andVictor W.M. van Hinsbergh Chapter 2 Isolation of Mouse Cardiac Microvascular Endothelial Cells Ralf P. Brandes, Stefani Beer, and Alexandra Aicher Chapter 3 Isolation,Culture, and Differentiation of Circulating Endothelial Precursor Cells Carmen Urbich and Stefanie Dimmeler Chapter 4 Embryonic Stem Cell-Derived Endothelial Cells Jun Yamashita and Shin-Ichi Nishikawa Chapter 5 Detection, Isolation, and Culture Of Lymphatic Endothelial Cells Taija Mäkinen and Kari Alitalo Chapter 6 Three-Dimensional Spheroid Culture of Endothelial Cells Thomas Korff Chapter 7 Immortalization of Endothelial Cells Yvonne Reiss and Friedemann Kiefer Chapter 8 Viral Gene Transfer Into Endothelial Cells Christoph Volpers and Stefan Kochanek Part II: Functional Assays in Vitro Chapter 9 Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress Olaf Zille, Markus Hammel, and Rupert Hallmann Chapter 10 Application of Biomechanical Forces on Endothelial Cells Andreas Schubert and Henning Morawietz Chapter 11 Endothelial Cell Permeability Assays in Culture Maria Grazia Lampugnani and Elisabetta Dejana Chapter 12 Three-Dimensional in Vitro Angiogenesis Assays Thomas Korff Chapter 13 Rat Aortic Ring Assay of Angiogenesis Roberto F. Nicosia and Wen-Hui Zhu Chapter 14 Endothelial Cell Chemotaxis and Chemokinesis Assays Hellmut G.Augustin Chapter 15 FACS Analysis of Endothelial Cells Yvonne Reiss and Britta Engelhardt Chapter 16 Application of the RNA Interference (RNAi) Technology to Angiogenesis Research Sung-Suk chae, Ji-Hye Paik, Jonathan Shubert-Coleman, Henry Furneaux, andTimothy Hla Part III: Functional Assays in Vivo Chapter 17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment Toshinori Murayama,Oren M. Tepper, and Takayuki Asahara Chapter 18 Balloon Denudation of Blood Vessels Volkhard Lindner Chapter 19 Peripheral Hind Limb Ischemia Models Stephan Schirmer, Imo Hoefer, and Ivo Buschmann Chapter 20 In Vivo Matrigel Angiogenesis Assays Tony Passaniti and Michele I.Vitolo Chapter 21 Analysis of Vascular Permeability in vivo Katja Issbrücker,Hugo H.Marti, and Matthias Clauss Chapter 22 Validating the Manipulation of Endothelial Cell signaling in Vivo John S. Rudge and Gavin Thurston Chapter 23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis Martin Hagedorn and Jörg Wilting Chapter 24 Corneal Angiogenesis Assay Lucia Morbidelli, Silvia Cantara, and Marina Ziche Part IV: Detection of Endothelial Cells Chapter 25 Basic Blood Vessel Immunohistochemistry Cynthia Obobodozie and Jens Kroll Chapter 26 Double-Staining Immunohistochemistry of Blood Vessels Valentin Goede Chapter 27 In Situ Hybridization Analysis of Vascular Endothelium Regina Heidenreich and Georg Breier Chapter 28 High-Resolution in Situ Confocal Analysis of Endothelial Cells Holger Gerhardt and Christer Betsholtz Chapter 29 Whole Mount Analysis of the Embryonic Vasculature Jody J.Haigh and Andras Nagy Chapter 30 Methods for Visualizing Intact Blood Vessels Gavin Thurston and Danielle Jean-Guillaume Chapter 31 Intravital Multi-Fluorescence Microscopy Mohammad Farhadi, Karsten Schwinn, and Peter Vajkoczy Chapter 32 Corrosion Cast Analysis of Blood Vessels Valentin Djonov and Peter H.