Technical advancements are basic elements in our life. In biophysical studies, new applications and improvements in well-established techniques are being implemented every day. This book deals with advancements produced not only from a technical point of view, but also from new approaches that are being taken in the study of biophysical samples, such as nanotechniques or single-cell measurements. This book constitutes a privileged observatory for reviewing novel applications of biophysical techniques that can help the reader enter an area where the technology is progressing quickly and where a comprehensive explanation is not always to be found.

Autorentext

Drs. Arrondo and Alonso are Professors in Biochemistry and Molecular Biology at the University of the Basque Country, which includes the Chair of Biophysics. Dr. Alonso has been teaching biophysics for more than 10 years and Dr. Arrondo for 5 years. Both are also members of the "Unidad de Biofisica", a joint research institute between the University of the Basque Country and the Spanish Research Council (CSIC), which they have headed for different periods.

Inhalt
Protein Nanocrystallization.- Differential Scanning Calorimetry of Proteins: an Overview and Some Recent Developments.- IR Reflectance Absorbance Studies of Peptide Structure, Orientation, and Conformational Flexibility in Langmuir Films: Relevance for Models of Pulmonary Surfactant Action.- Two-Dimensional Infrared Correlation Spectroscopy.- NMR Spectroscopy of Large Biological Macromolecules in Solution.- Emerging Techniques in Fast Multidimensional NMR.- Principles and Application of Projected Multidimensional NMR Spectroscopy G-matrix Fourier Transform NMR.- Protein Nanomechanics as Studied by AFM Single-Molecule Force Spectroscopy.- Multiphoton-Excitation Fluorescence Microscopy and Membranes.- Three-Dimensional Electron Microscopy. The Coming of Age of a Versatile Structural Biology Technique.