Human Embryonic Stem Cells

With this valuable practical guide, three members of the Harvard
Stem Cell Institute have compiled and edited the definite handbook
for the exciting new field of human embryonic stem cell research.
The editors have gathered protocols from scientists with extensive
reputation and expertise, describing and comparing currently used
techniques for the culture of human stem cells and discussing the
strengths and weaknesses of the different approaches.

Human Embryonic Stem Cells: The Practical
Handbook contains the first centralised collection of
methods used in human embryonic stem cell biology. The book covers
the derivation of human stem cell lines, the obtaining of cells
from human stem cell banks, the culturing and characterisation of
the cells, and the differentiation of the cells in vitro and
in vivo. Lastly, almost all of these protocols can also be
used for analyzing and manipulating induced pluripotency iPS stem
cells. This allows an even greater number of opportunities for
those interested in purusing work in pluripotent stem cells,
disease modelling, and other aspects of basic regenerative medicine
research.

The novel and useful focus of this book sets it apart from other
available books:

• Compares and evaluates the protocols used in leading
  laboratories working on human embryonic stem cells

• Centred solely on practical protocols for human (not mouse)
  embryonic stem cell research

• Includes extensive troubleshooting sections

• Addresses the different proclivities and behaviours of
  individual human embryonic cell lines

• Contains techniques currently known only to a small number of
  specialised laboratories worldwide

This handbook represents an essential source of up-to-date
practical information for all cell and developmental biologists
working with human embryonic stem cells or wishing to enter the
field. It is also essential reading for clinical researchers
in areas such as diabetes, cardiovascular disease, and neurological
diseases.

Praise from the reviews:

"...a highly readable and useful book... A notable
feature of the book is its air of openness and honesty... This
book... will help many to navigate the uncharted waters of
human embryonic stem cell biology." BRITISH SOCIETY FOR CELL
BIOLOGY

"... the imaginative solutions in this book can inspire us to
get past our most frustrating limitations." CELL STEM CELL

"... the richness in the details of each protocol presented
will certainly encourage more scientists to begin studies of Human
pluripotent stem cells..." REGENERATIVE MEDICINE

"In this fast-moving field, this [handbook] will help drive
advances of more and more researchers."
DIFFERENTIATION

"...a valuable resource for seasoned and novice
researchers... an excellent addition to the reference collection of
any medical library or research laboratory." THE AMERICAN
MEDICAL ASSOCIATION

Autorentext
Stephen Sullivan: Trinity College, Dublin, Ireland Kevin Eggan: Harvard University, Cambridge, MA, USA

Chad A. Cowan: Harvard University, Cambridge, MA, USA

Klappentext
Since their isolation in 1998, human embryonic stem (ES) cells have been used as a powerful experimental model for studying the mechanisms of stem cell self-renewal, pluripotency, and differentiation in humans. However, the protocols by which these cells are grown still differ considerably from laboratory to laboratory and standardisation is needed in this young area of research. Human Embryonic Stem Cells: The Practical Handbook contains all basic methods for sourcing, deriving, culturing, and manipulating human ES cells. The editors have gathered the leading scientists in the field to describe the protocols that they have carried out and tested in their own labs.

The book

• Contains practical information for newcomers to the field: from how to source human ES cells from international cell banks to organizing the laboratory to accommodate work with these cells.

• Provides protocols for routine culture as well as specialized protocols for directing differentiation into specific cell lineages.

• Addresses the different proclivities and behaviours of individual human ES cell lines: authors state which lines they tried their protocols on and, more importantly, which lines worked best.

• Includes troubleshooting sections that address common problems and offer advice on how these can be overcome.

• Follows a simple, concise, and consistent structure: background material and hypothesizing are kept to a minimum to insure clarity and usability at the bench.
  Human Embryonic Stem Cells: The Practical Handbook is the essential source of up-to-date, practical information for all researchers and clinicians working with human embryonic stem cells.

Zusammenfassung
With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in purusing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research.

The novel and useful focus of this book sets it apart from other available books:

• Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells
• Centred solely on practical protocols for human (not mouse) embryonic stem cell research
• Includes extensive troubleshooting sections
• Addresses the different proclivities and behaviours of individual human embryonic cell lines
• Contains techniques currently known only to a small number of specialised laboratories worldwide
  This handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases.

Praise from the reviews:

"...a highly readable and useful book A notable feature of the book is its air of openness and honesty This book will help many to navigate the uncharted waters of human embryonic stem cell biology." BRITISH SOCIETY FOR CELL BIOLOGY

"... the imaginative solutions in this book can inspire us to get past our most frustrating limitations." CELL STEM CELL

"... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE

"In this fast-moving field, this [handbook] will help drive advances of more and more researchers." DIFFERENTIATION

"...a valuable resource for seasoned and novice researchers... an excellent addition to the reference collection of any medical library or research laboratory." THE AMERICAN MEDICAL ASSOCIATION

Inhalt
Foreword. Davor Solter Preface.

Section 1: Obtaining and culturing human e…