This book constitutes the proceedings of the 16th IFIP WG 11.12 International Symposium on Human Aspects of Information Security and Assurance, HAISA 2022, held in Mytilene, Lesbos, Greece, in July 2022. The 25 papers presented in this volume were carefully reviewed and selected from 30 submissions. They are organized in the following topical sections: cyber security education and training; cyber security culture; privacy; and cyber security management.