Cell Technology for Cell Products

The 19th ESACT meeting was to highlight the novel capabilities of the industry to move the products towards the clinic. It was attended by a wide range of workers in the industry and for many it was their first ESACT meeting. The proceedings here include the short papers adding the knowledge of the previous meetings and provide a reference for the researcher entering, or continuing in the field of Animal Cell Technology.

Zusammenfassung

The 19th ESACT meeting was to highlight the novel capabilities of the industry to move the products towards the clinic and was attended by a wide range of workers in the industry and for many it was their first ESACT meeting. The meeting was started with a session on Transcription to Secretion with a notable set of presentations on the emerging issues. The other sessions that followed Therapeutic Cell Engineering, Gene Medicine, Cells to Tissue, Protein products and Process Technology guided the delegates through the advances made for the progression of the biotechnology towards the industrial application of the products from cells. The meeting was supported by some exceptional invited speakers from around the world whose contributions complemented the emerging technologies and the changes being made at the industrial end of the ESACT spectrum. The proceedings here include the short papers adding the knowledge of the previous meetings and provide a reference for the researcher entering, or continuing in the field of Animal Cell Technology.

Inhalt

Contents; 19th ESACT Meeting Committees; ESACT Executive Committee; Sponsors; Companies in Trade Exhibition; Acknowledgements; List of Participants; CHAPTER I: PERSPECTIVES OF THE BIOTECH INDUSTRY; The UK bioprocessing sector, Tony Bradshaw; CHAPTER II: TRANSCRIPTION TO SECRETION; A Novel AIR-induced Gene Expression System in HEK.EBNA Cells and its Applications, N.S. Werner et al.; Towards Stronger Gene Expression - a Promoter's Tale, S. Kalwy et al.; Improving the Expression of a Soluble Receptor: Fc fusion Protein in CHO Cells by Coexpression with the Receptor Ligand, G.W. Lee et al.; Growth, Apoptosis and Functional Genomics Analysis of CHO-K1 Over-Expressing Telomerase, F. Crea et al.; Microarray Analysis of Metabolically Engineered NS0 Cell Lines Producing Chimeric Antibody, G. Khoo et al.; Optimizing Medium for Transient Transfection, Z.-H. Geng et al.; Establishment of Recombinant CHO Cell Lines Under Serum-free Conditions, M. Kuchenbecker et al.; Novel Cell-based Assay for the Detection of Murine type I IfN, M. Bollati-Fogolín et al.; Characterisation of the Induction of Cytochrome p450 Enzymes in Primary Cultures of Human Hepatocytes, T.L. Freeman et al.; Effect of Culture Conditions on Glycosylation of Recombinant beta-Interferon in CHO Cells, M. Spearman et al.; Induction of Caspase-3-dependent Apoptosis by Electrolyzed Reduced Water/platinum Nanoparticles in Cancer Cells, T. Hamasaki et al.; Molecular Mechanism of TAK1-Induced Repression of hTERT Transcription, M. Maura, et al.; Analysis of Genetic Parameters in Order to Get More Information on High Producing Recombinant CHO Cell Lines, C. Lattenmayer et al.; Redox Regulation by Reduced Waters as Active Hydrogen Donors and Intracellular ROS Scavengers for Prevention of type 2 Diabetes, S. Shirahata et al.; Transcription Profiling of Different Recombinant CHO Clones Based on Cross-species Microarray Analysis, E. Trummer et al.; Adaptation without Cell Division? K.L. Astley et al.; Release ofPlasmid-DNC from PEI/DNA Particles, M. Bertschinger et al.; Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody, S. Matsumoto et al.; Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody, C.A. Caldas et al.; Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1, D.L. Hacker et al.; The Role of the Sumoylation Pathway in Transient and Stable Recombinant Protein Expression in Chinese Hamster Ovary Cells, M. Bertschinger et al.; Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium, P. Aizawa et al.; Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods, A.J. Porter et al.; Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells, M. Schomberg et al.; Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration, J. Rodríguez et al.; Metabolic Changes Occuring During Adaptation to Serum Free Media, S. Denby et al.; The Expression of the neo gene in GS-NS0 Cells Increases their Proliferative Capacity, F. Veraitch et al.; CHAPTER III: THERAPEUTIC CELL ENGINEERING; The Therapeutic Potential of Human Embryonic Stem Cells, S.H. Cedar et al.; Investigations of Murine Embryonic Stem Cell Maintenance by Analyses of Culture Variables and Gene Expression, J.M. Piret et al.; Generation of Cardiomyocytes Derived from Mouse ESC in a Scale-up System, S. Niebruegge et al.; Expansion of Undifferentiated Human Embryonic Stem Cells, A.B.H. Choo et al.; New Electrofusion Devices for the Improved Generation of Dendritic Cell-tumour Cell Hybrids, J. Schaper et al.; Influence of c-Myc and bcl-2 CO-expression on Proliferation, Apoptosis and Survival in CHO Cultures, V. Ifandi et al.; Ex Vivo Expansion of Hematopoietic Stem Cells Using Defined CultureMedia, J. Hartshorn et al.; Development of A Novel Serum-free Cryopreservative Solution, T. Toyosawa et al.; Hepatic Cell Lines CultureD on Different Scaffolds and in Different Stages for Bioartificial Liver Systems, K. Kaito et al.; Comparison of Flask and Bag Systems in Cultivation of Dendritic Cells for Clinical Application, L. Macke et al.; T-Lymphocytes Transduced with SFCMM-3 Vector in a Closed System, C. Benati et al.; Influence of Down-regulation of Caspase-3 and CASPASE-7 by Sirnas on Sodium Butyrate-induced Apoptotic Cell Death of Chinese Hamster Ovary Cells Producing Thrombopoietin, Y.H. Sung et al.; CHAPTER IV: GENE MEDICINE; UTRtech(TM): Exploiting mRNA Targeting To Increase Protein Secretion From Mammalian Cells, B. Stern et al.; Semi-Synthetic Mammalian Gene Regulatory Networks, B.P. Kramer et al.; Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention, V.M. Gorenflo et al.; Comparison of Different Transient Retroviral Transfection Systems, V. Kleff et al.; CHAPTER V: CELLS TO TISSUES; Artificial Human Lymphnode, C. Giese et al.; Single Cell Study in a Hydrogel, T. Braschler et al.; Predicting Tissue Assembly of Prostate Cancer Spheroids, K. O'Connor et al.; Cultivating Cells of Different Origin for 3d Bone Constructs Considering Physiological Conditions, K. Suck et al.; Guidance on Good Cell Culture Practice, S. Coecke et al.; In Vitro Vascularization of Human Microtissues, J.M. Kelm et al.; Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture, N. Nagai et al.; Propagation of USSC for Therapeutic Approaches, J. Lamlé et al.; Bioreactor Based Production of Functional Conditioned Medium for the Propagation of Undifferentiated Human Embryonic Stem Cells, A. Bauwens et al.; Large-scale Expansion of Cells for Use as Therapeutic Agents, J. Melero-Martin et al.; EX VIVO Expansion of CD34+ Cells in Stemline. I I Hematopoietic Stem Cell Expansion Medium Generates a Large Population of FunctionalEarly and Late Progenitor Cells, S.L. Leugers et al.; In Vitro Bone Formation of Multipotential Human Mesenchymal Cells in Fixed Bed Perfusion Bioreactors, N. Zghoul et al.; Influence of Basal Media Formulations and Calcium on Growth and Directed Differentiation of Human Osteogenic Cells In Vitro, I. Majore et al.; CHAPTER VI: PROTEIN PRODUCTS; BHRF-1 as a Tool for Genetic Inhibition of Apoptotis in Hybridoma Cell Cultures, S. Juanola et al.; Karyotype of CHO DG44 cells, D. Martinet et…