Weltweit führende Experten präsentieren den heutigen Stand des Wissens auf dem Gebiet der Mikrobiologie der Rhizosphäre. Der Schwerpunkt liegt hierbei auf den aktuellen Methoden und Strategien zur Entdeckung, Identifizierung und Kontrolle von genetisch veränderten Mikroorganismen (GVOs) in der engsten Umgebung von lebenden Pflanzenwurzeln.

Wichtige Inhalte sind:

Züchtung genetisch markierter Rhizosphären-Bakterien - Entdeckung markierter genetisch veränderter Organismen (GVOs) - Identifizierung solcher GVOs mittels DNA-Sonde und PCR Verstärkung
Schnellunterscheidung von genetisch veränderten und nicht genetisch veränderten Organismen mit PCR-gestützten Techniken - Risikoabwägung beim Gentransfer
EG Richtlinien für den Gebrauch und die Freisetzung von GVOs - Ergebnisse von Felduntersuchungen zur Sicherheit von Freisetzungsexperimenten

Autorentext

Fergal O'Gara is the author of Molecular Ecology of Rhizosphere Microorganisms: Biotechnology and the Release of GMOs, published by Wiley.

David N. Dowling is the author of Molecular Ecology of Rhizosphere Microorganisms: Biotechnology and the Release of GMOs, published by Wiley.

Bert Boesten is the author of Molecular Ecology of Rhizosphere Microorganisms: Biotechnology and the Release of GMOs, published by Wiley.

Zusammenfassung
This book helps evaluate the state of the art of rhizosphere microbial ecology and biotechnology. Experts in the field review methods and strategies applied to the detection, identification and monitoring of microorganisms in the rhizosphere. Major topics treated include:

construction of genetically marked rhizosphere bacteria - detection of marked wildtype and genetically modified organisms (GMOs)
identification of wildtype and GMOs by DNA probes and PCR amplification
rapid typing of non-modified and GMOs by PCR-based techniques - assessment of the role of gene transfer
EU regulations for the use and release of GMOs
biosafety results from field testing of GMOs
In addition, technologies for the modifying gene expression and gene products for specific traits of agronomic interest in genetically engineered rhizosphere bacteria are covered.

Inhalt

Aus dem Inhalt:
Current Challenges in Introducing Beneficial Microorganisms into the Rhizosphere (D. M. Weller, L. S. Thomashow)/ Studies on Indigenous Endophytic Bacteria of Sweet Corn and Cotton (J. A. McInroy, J. W. Kloepper)/ Detection of Introduced Bacteria in the Rhizosphere Using Marker Genes and DNA Probes (M. H. Ryder, C. E. Pankhurst, A. D. Rovira, R. L. Correll)/ Impact of GEMMOs on Rhizosphere Population Dynamics (J. M. Lynch, F. A. A. M. de Leij, J. M. Whipps, M. J. Bailey)/ Developing Concepts in Biological Control: A Molecular Ecology Approach (D. N. Dowling, B. Boesten, P. R. Gill, Jr. and F. O'Gara)/ Biocontrol of Root Diseases by Pseudomonas fluorescens CHA0: Current Concepts and Experimental Approaches (C. Voisard et al.)/ Genetic Strategies to Engineer Expression Systems Responsive to Relevant Environmental Signals (V. de Lorenzo)/ Genetic Typing of Microorganisms: Current Concepts and Future Prospects (H.-V Tichy, R. Simon)/ Development of Subtraction Hybridization Procedures for Generating Strain-Specific Rhizobium DNA Probes (J. E. Cooper, A. J. Bjourson)/ Molecular Characterization and Detection of the Actinomycete Frankia in the Environment (P. Simonet, S. Nazaret, P. Normand)/ Molecular Ecology of Filamentous Actinomycetes in Soil (P. Marsh, E. M. H. Wellington)/ Some Considerations on Gene Transfer between Bacteria in Soil and Rhizosphere (J. D. von Elsas, E. Smit)/ European Community Regulation for the Use and Release of Genetically Modified Organisms (GMOs) in the Environment (M. P. Nuti, A. Squartini, A. Giacomini)/ Index