Antennas and Reaction Centers of Photosynthetic Bacteria

The workshop on "Antennas and Reaction Centers of Photosynthetic Bac­ teria" was held at Feldafing, Bavaria (F. R. G. )' March 23-25, 1985. This workshop focussed on primary processes with emphasis on structure, inter­ actions and dynamics. It assessed structural, spectroscopic and dynamic data which have accumulated recently, providing an overview of the mech­ anism of the acquisition, storage and useful disposal of energy in bacterial photosynthesis. This volume is a record of the invited papers presented at the workshop. The material was organized into five sections: I. Antennas: Structure and Energy Transfer II. Reaction Centers: Structure and Interactions III. Electron Transfer: Theory and Model Systems IV. Reaction Cen,ters: Structure and Dynamics V. Model Systems on Function of Antennas and Reaction Centers I would like to express my gratitude to all the participants in the work­ shop for their contributions, and to the authors for the timely preparation of their manuscripts. I am indebted to the members of the organizing committee, Professors Sighart F. Fischer and Hugo Scheer for their most valuable assistance and advice. The workshop would not have been so successful without the help of my secretary, Frau Petra KahlfuB, and my coworkers in its organization. I thank Frau KahlfuB particularly also for her assistance in the preparation of these proceedings. The workshop was organized under the auspices of the Technical Uni­ versity of Munich, the Max-Planck-Society and the University of Munich.

Inhalt

I Antennas: Structure and Energy Transfer.- Structure of Antenna Polypeptides.- The Crystal and Molecular Structure of C-Phycocyanin.- C-Phycocyanin from Mastigocladus laminosus. Isolation and Properties of Subunits and Small Aggregates.- Picosecond Time-Resolved, Polarized Fluorescence Decay of Phycobilisomes and Constituent Biliproteins Isolated from Mastigocladus laminosus.- Fluorescence Behaviour of Crystallized C-Phycocyanin (Trimer) from Mastigocladus laminosus.- Energy-Transfer Kinetics in Phycobilisomes.- Exciton State and Energy Transfer in Bacterial Membranes: The Role of Pigment-Protein Cyclic Unit Structures.- Carotenoid-Bacteriochlorophyll Interactions.- Bacteriochlorophyll a- and c-Protein Complexes from Chlorosomes of Green Sulfur Bacteria Compared with Bacteriochlorophyll c Aggregates in CH2C12-Hexane.- Reverse-Phase High-Performance Liquid Chromatography of Antenna Pigment- and Chlorosomal Proteins of Chloroflexus aurantiacus.- Fluorescence-Detected Magnetic Resonance of the Antenna Bacteriochlorophyll Triplet States of Purple Photosynthetic Bacteria.- High-Resolution 1H NMR of Light-Harvesting Chlorophyll- Proteins.- Crystallization and Linear Dichroism Measurements of the B800- 850 Antenna Pigment-Protein Complex from Rhodopseudomonas sphaeroides 2.4.1.- Crystallization of the B800-850-complex from Rhodopseudomonas acidophila Strain 7750.- Linear Dichroism (LD) and Absorption Spectra of Crystals of B800-850 Light-Harvesting Complexes of Rhodopseudomonas capsulata.- II Reaction Centers: Structure and Interactions.- The Crystal Structure of the Photosynthetic Reaction Center from Rhodopseudomonas viridis.- Single Crystals from Reaction Centers of Rhodopseudomonas viridis Studied by Polarized Light.- On the Analysis of Optical Spectra of Rhodopseudomonas viridis Reaction Centers.- Orientation of the Chromophores in the Reaction Center of Rhodopseudomonas viridis. Comparison of Low-Temperature Linear Dichroism Spectra with a Model Derived from X-Ray Crystallography.- Calculations of Spectroscopic Properties of Bacterial Reaction Centers.- On the Temperature-Dependence of the Long Wavelength Fluorescence and Absorption of Rhodopseudomonas viridis Reaction Centers.- Local Environments of Pigments in Reaction Centers of Photosynthetic Bacteria from Resonance Raman Data.- The Spin-Polarization Pattern of the ?m = 1 Triplet EPR Spectrum of Rps. viridis Reaction Centers.- Triplet State Investigation of Charge Separation and Symmetry in Single Crystals of R. viridis Reaction Centers.- Triplet-minus-Singlet Absorbance Difference Spectroscopy of Photosynthetic Reaction Centers by Absorbance-Detected Magnetic Resonance.- ENDOR Studies of the Primary Donor in Bacterial Reaction Centers.- ENDOR of Semiquinones in RCs from Rhodopseudomonas sphaeroides.- Photoinduced Charge Separation in Bacterial Reaction Centers Investigated by Triplets and Radical Pairs.- Spin Dipolar Interactions of Radical Pairs in Photosynthetic Reaction Centers.- Protein/Lipid Interaction of Reaction Center and Antenna Proteins.- The Architecture of Photosystem II in Plant Photosynthesis. Which Peptide Subunits Carry the Reaction Center of PS II?.- III Electron-Transfer: Theory and Model Systems.- Application of Electron-Transfer Theory to Several Systems of Biological Interest.- Effects of Distance, Energy and Molecular Structure on Long- Distance Electron-Transfer Between Molecules.- Ultrafast Electron Transfer in Biomimetic Models of Photosynthetic Reaction Centers.- Electron Transfer Through Aromatic Spacers in Bridged Electron- Donor-Acceptor Molecules.- Electron Transfer in Rigidly Linked Donor-Acceptor Systems.- Electron Conduction Along Aliphatic Chains.- IV Reaction Centers: Structure and Dynamics.- Kinetics and Mechanisms of Initial Electron-Transfer Reactions in Rhodopseudomonas sphaeroides Reaction Centers.- Femtosecond Studies of the Reaction Center of Rhodopseudomonas viridis: The Very First Dynamics of the Electron-Transfer Processes.- Analysis of Time-resolved Fluorescence of Rhodopseudomonas viridis Reaction Centers.- The Characterization of the QA Binding Site of the Reaction Center of Rhodopseudomonas sphaeroides..- V Model Systems on Structure of Antennas and Reaction Centers.- Structure and Energetics in Reaction Centers and Semi-synthetic Chlorophyll Protein Complexes.- Small Oligomers of Bacteriochlorophylls as in vitro Models for the Primary Electron Donors and Light-Harvesting Pigments in Purple Photosynthetic Bacteria.- Experimental, Structural and Theoretical Models of Bacteriochlorophylls a, d and g..- ENDOR Characterization of Hydrogen-Bonding to Immobilized Quinone Anion Radicals.- Concluding Remarks. Some Aspects of Energy Transfer in Antennas and Electron Transfer in Reaction Centers of Photosynthetic Bacteria.- Index of Contributors.