Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses

On May 4-8, 1987, a NATO Advanced Research Workshop on the Analytical Uses of Immobilized Biological Compounds was held in Florence, Italy. The Director of the Workshop was Professor George G. Guilbault of the University of New Orleans, and the Co-Director was Professor Marco Mascini of the University of Florence It vas the purpose of this meeting to assemble scientists from all NATO Countries with an interest in immobilized biological compounds. to discuss - methods of immobilization - properties of immobilized compounds - enzyme electrodes and biosensors - optical devices utilizing immobilized enzymes - microbial sensors and clinical uses of immobilized enzymes - flow injection analysis using enzymes - immobilized biological compounds in chemical defense detection - pharmaceutical analysis - uses in industrial analysis - enzyme reactors - air pollution detectors - immunosensors - medical uses and applications - solid state and FET sensors Goals to be achieved by the conference were - to permit an exchange of views and experience in all these areas - to review and critically assess the state-of-the-art in these fields - to set guidelines for future research and establish collaborative projects between scientists in NATO laboratories in the above areas. Thirty-seven lectures were given by 36 speakers in all of the above areas.

Inhalt

A. Immobilization Techniques - General.- Enzymes Immobilized on Inorganic Supports.- Immobilization of Bioagents by Radiation.- Structural and Transport Properties of Polymeric Carriers for Bioagents and Immobilization Techniques and Their Applications.- B. Clinical and Pharmaceutical Applications.- Immobilized Enzymes in Therapy.- Uses of Immobilized Biological Compounds in Pharmaceutical Analysis.- Application of Immobilized Enzymes in Diagnostics.- Immobilized Dehydrogenase Enzymes Coupled with a NADH Sensor.- Targeting of Immobilized Drugs.- C. Biosensors.- Enzyme and Microbial Sensor.- Redox Mediators and Their Application in Amperometric Sensors.- Uni-, Bi- and Tri-Enzyme Electrodes for Analysis.- Enzyme Electrochemical Sensors for "In Vivo" Analysis with Artificial Pancreas.- Biosensors in Clinical and Pharmaceutical Analysis.- Graft Copolymers as Supports for the Immobilization of Biological Compounds.- D. Defense Applications.- Use of Protein Coatings on Piezoelectric Crystals for Assay of Gaseous Pollutants.- E. Fet Detectors.- Use of Immobilized Enzymes in FET Detectors.- Micro-Biosensor.- F. Optrodes - Spectroscopy.- The Development of Fibre-Optic Sensors by Immobilization of Fluorescent Probes.- Developing Biosensors.- Immobilized Biological Compounds in Bio- and Chemiluminescence Assays.- Immobilized Biospecific Proteins in Analytical Clinical Chemistry.- G. Immunosensors.- Immunosensors.- Electrochemical Immunosensors.- H. Flow Analysis.- Flow Injection Analysis - Recent Developments and Future Trends.- Extension to Enzymatic Electrodes of a General Procedure for the Construction of Flow-Through ISE's Based on the Use of Conductive Epoxy as Support of the Sensor.- I. Industrial and Analytical Applications.- Biosensor-Based Analyzers; From Design to Instrument.- Commercial Aspects of the Use of Immobilized Compounds.- Design Considerations of an Immobilized Enzyme Electrode for Measurement of Glucose in Whole Blood.- Diffusion Limited Enzyme Electrodes.- Enzyme Electrodes for Sugar Substitute Aspartame.- Author Index.