Advanced Fragmentation Methods in Biomolecular Mass Spectrometry

Breaking down large biomolecules into fragments in a controlled manner is key to modern biomolecular mass spectrometry. This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods. It provides a comprehensive overview of their history, mechanisms, instrumentation, and key applications. With contributions from leading experts, this book will act as an authoritative guide to these methods.
Aimed at postgraduate and professional researchers, mainly in academia, but also in industry, it can be used as supplementary reading for advanced students on mass spectrometry or analytical (bio)chemistry courses.

Autorentext

Frederik Lermyte is an Assistant Professor of Conformation-Sensitive Mass Spectrometry at the Technical University of Darmstadt. He studied Chemistry at the University of Antwerp, and completed his PhD in 2016. His PhD work focused on the use of ion mobility spectrometry and advanced (predominantly electron-based) fragmentation methods in structural biology. He subsequently worked as a Research Fellow at the University of Warwick for three years, where he used ultrahigh-resolution tandem MS and synchrotron spectromicroscopy to study the role of metals in neurodegeneration, and as a postdoctoral researcher at the University of Liège, before moving to Darmstadt. His research interests include fundamental and applied aspects of native MS, top-down protein analysis, and ion mobility spectrometry.

Inhalt

Modern Mass Spectrometry and Advanced Fragmentation Methods; Mechanism and Implementation of Electron Capture and Electron Transfer Dissociation; ECD/ETD for Sequencing of Peptides and Denatured Proteins; Structural Studies Using Electron-Based Fragmentation Methods and Chemical Labelling of Proteins; Investigating 3D Structures of Native Proteins and Complexes through Electron-Based Dissociation; Electron Detachment Dissociation (EDD) and Negative Electron Transfer Dissociation (NETD); Electron Photodetachment and the Radical Fragmentation Route for Negative Ions; Elucidating Biomolecular Structure through Bond-Selective Radical Fragmentation; Structural Analysis of Lipids Using Advanced Tandem MS Methods; Photoactivation and Dissociation in Biomolecular Mass Spectrometry; Surface-induced Dissociation in Biomolecular Mass Spectrometry