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Confidently face the challenges of proteomics research specific to plant science with the information in Plant Proteomics, which will introduce you to the techniques and methodologies required for the study of representative plant species. Read about proteomics studies in Arabidopsis, rice, and legumes and find information about common technologies like mass spectrometry and gel electrophoresis. Discover expression proteomics, functional proteomics, structural proteomics, bioinformatics, and systems biology, understand how to conduct proteomics studies in developing countries and underfunded laboratories, and gain access to guidelines for sample preparation.
Auteur
Ganesh Kumar Agrawal, PhD, is the Associate Director of the
Research Laboratory for Biotechnology and Biochemistry (RLABB) in
Kathmandu, Nepal. He earned his doctorate in applied biological
chemistry at the Tokyo University of Agriculture and Technology in
Randeep Rakwal, PhD, is presently a researcher on the
Tsukuba Mental Stress Team at the Health Technology Research Center
(HTRC), National Institute of Advanced Industrial Science and
Technology (AIST) in Japan. He earned his doctorate in applied
biological chemistry at the Tokyo University of Agriculture and
Technology in 1997. His current research focuses on the genomics,
proteomics, and metabolomics of environmental stress responses in
plant and animal systems.
Texte du rabat
Where will plant proteomics take us in the near future?
Plant Proteomics: Technologies, Strategies, and Applications answers these questions and more. Plant proteomics has great potential for improving yield and disease resistance as well as for developing species that better satisfy the requirements of human and animal nutrition. This reference provides a broad perspective on the state of plant proteomics and delineates exciting advances in the discipline. With contributions from leading researchers worldwide, it:
Describes proteomics studies in Arabidopsis, rice, and legumes
Includes information on using common technologies such as mass spectrometry and gel electrophoresis, and previews emerging technologies
Discusses expression proteomics, functional proteomics, structural proteomics, bioinformatics, and systems biology
Has chapters dealing with the specific proteomes of plant tissues, such as seed, root, anther, and leaf, all containing important organelles such as chloroplasts and mitochondria
Provides guidelines for sample preparation
Includes abbreviations, glossaries, and resources for further information
Focusing exclusively on plant proteomics, this is the premier reference for scientists conducting plant proteomics research and for students in plant sciences.
Contenu
PREFACE.
CONTRIBUTORS.
ACRONYMS AND ABBREVIATIONS.
PART I: TECHNOLOGIES.
GEL-BASED PROTEOMICS (Pier Giorgio Righetti, Paolo Antonioli, Carolina Simo, and Attilio Citterio).
MASS SPECTROMETRY-BASED PROTEOMICS: IDENTIFYING PLANT PROTEINS (Eveline Bergmuller, Sacha Baginsky, and Wilhelm Gruissem).
CHEMICAL PROTEOMICS (Miriam C. Hagenstein, Olaf Kruse, and Norbert Sewald).
THE ARABIDOPSIS LOCALIZOME: SUBCELLULAR PROTEIN LOCALIZATION AND INTERACTIONS IN ARABIDOPSIS (Georgios Kitsios, Nicolas Tsesmetzis, Max Bush, and John H. Doonan).
SECRETOME: TOWARD DECIPHERING THE SECRETORY PATHWAYS AND BEYOND (Young-Ho Jung, Ganesh Kumar Agrawal, Randeep Rakwal, and Nam-Soo Jwa).
PEPTIDOMICS (Peter Schulz-Knappe).
PART II: COMPUTATIONAL PROTEOMICS.
BIOINFORMATICS IN GEL-BASED PROTEOMICS (Asa M. Wheelock and Craig E. Wheelock).
BIOINFORMATICS IN MS-BASED PROTEOMICS (Jacques Colinge).
PART III: EXPRESSION PROTEOMICS.
AN OVERVIEW OF THE ARABIDOPSIS PROTEOME (Jacques Bourguignon and Michel Jaquinod).
RICE PROTEOME AT A GLANCE (Ganesh Kumar Agrawal and Randeep Rakwal).
PROTEOMICS OF LEGUME PLANTS (Satish Nagaraj, Zhentian Lei, Bonnie Watson, and Lloyd W. Sumner).
PROTEOME OF SEED DEVELOPMENT AND GERMINATION (Julie Catusse, Loc Rajjou, Claudette Job, and Dominique Job).
ENDOSPERM AND AMYLOPLAST PROTEOMES OF WHEAT GRAIN (William J. Hurkman, William H. Vensel, Frances M. DuPont, Susan B. Altenbach, and Bob B. Buchanan).
ROOT PROTEOME (Kuo-Chen Yeh, Chyi-Chuann Chen, and Chuan-Ming Yeh).
LEAF PROTEOME (Bin Kang, Shuyang Tu, Jiyuan Zhang, and Siqi Liu).
ANTHER PROTEOME (Nijat Imin).
POLLEN PROTEOME (Sandra Noir).
MICROTUBULE-BINDING PROTEINS (Lori A. Vickerman and Douglas G. Muench).
PART IV: ORGANELLE PROTEOMICS.
CELL WALL (Elisabeth Jamet, Herv´e Canut, Cecile Albenne, Georges Boudart, and Rafael Pont-Lezica).
PLASMA MEMBRANE: A PECULIAR STATUS AMONG THE CELL MEMBRANE SYSTEMS (Geneviève Ephritikhine, Anne Marmagne, Thierry Meinnel, and Myriam Ferro).
NUCLEUS (Subhra Chakraborty, Aarti Pandey, Asis Datta, and Niranjan Chakraborty).
CHLOROPLAST (Thomas Kieselbach and Wolfgang P. Schröder).
ETIOPLAST (Anne von Zychlinski, Sonja Reiland, Wilhelm Gruissem, and Sacha Baginsky).
THE PLANT MITOCHONDRIAL PROTEOME AND THE CHALLENGE OF HYDROPHOBIC PROTEIN ANALYSIS (Yew-Foon Tan and A. Harvey Millar).
PEROXISOME (Yuko Arai, Youichiro Fukao, Makoto Hayashi, and Mikio Nishimura).
UNRAVELING PLANT VACUOLES BY PROTEOMICS (Songqin Pan and Natasha Raikhel).
OIL BODIES (Pascale Jolivet, Luc Negroni, Sabine d'Andrea, and Thierry Chardot).
PART V: MODIFICATION PROTEOMICS.
PHOSPHOPROTEINS: WHERE ARE WE TODAY? (Florian Wolschin and Wolfram Weckwerth).
PROTEOME ANALYSIS OF THE UBIQUITIN PATHWAY (Junmin Peng).
ANALYSIS OF THE N-GLYCOSYLATION OF PROTEINS IN PLANTS (Willy Morelle).
FUNCTIONAL ANALYSIS AND PHOSPHORYLATION SITE MAPPING OF LEUCINE-RICH REPEAT RECEPTOR-LIKE KINASES (Steven D. Clouse, Michael B. Goshe, Steven C. Huber, and Jia Li).
TIME TO SEARCH FOR PROTEIN KINASE SUBSTRATES (Birgit Kersten).
TYROSINE PHOSPHORYLATION IN PLANTS: EMERGING EVIDENCE (Andrea Carpi, Valeria Rossi, and Francesco Filippini).
1433 PROTEINS: REGULATORS OF KEY CELLULAR FUNCTIONS (Peter C. Morris).
PART VI: MULTIPROTEIN COMPLEX.
TAP-TAGGING SYSTEM IN RICE FOR PROTEIN COMPLEX ISOLATION (Jai S. Rohila and Michael E. Fromm).
TAP STRATEGY IN ARABIDOPSIS PROTEIN COMPLEX ISOLATION (Vicente Rubio and Xing Wang Deng).
BLUE-NATIVE PAGE IN STUDYING PROTEIN COMPLEXES (Holger Eubel and A. Harvey Millar).
PROTEINPROTEIN INTERACTION MAPPING IN PLANTS (Joachim F. Uhrig).
PART VII: PLANT DEFENSE AND STRESS.