During the past 15 years, there has been remarkable progress in the analysis and manipulation of DNA and its use in nanotechnology. DNA analysis is ubiquitous in molecular biology, medical diagnostics, and forensics. Much of the readout technology is based on fluorescence detection. This volume contains contributions from many experts in the field who present an overview of many aspects of DNA technology. These chapters provide an understanding of the underlying principles and technology, rather than an exhaustive review of the literature. Written in a clear straightforward style, this book is an excellent introduction for any scientist to the use of fluorescence in DNA analysis.

DNA Technology is an essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field.

Key Features: *Comprehensive overview of the complexities of DNA analysis, *Covers topics of universal interest to a broad field of scientists, *Accessible utility in presenting state-of-the-art DNA technology, *Chapters authored by key figures in the field.

Auteur

Dr. J.R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the sole author of the widely used text, Principles of Fluorescence Spectroscopy, also published by Kluwer Academic/Plenum Publishers, now in its Second Edition.

Contenu

DNA Sequencing Using Fluorescence Detection.- Fluorescence in Nucleic Acid Hybridization Assays.- Energy Transfer Fluorescent Labels for DNA Sequencing and Analysis.- On-the-Fly Fluorescence Lifetime Detection in Capillary Electrophoresis for DNA Analysis.- Fluorescent Nucleoside Analogues as DNA Probes.- Lanthanide-Labeled DNA.- DNA Arrays for Genetic Analyses and Medical Diagnosis.- Flow Cytometric Sizing of DNA Fragments.- Fluorimetric DNA Biosensors.- Technicolor Genome Analysis.