Enzymes and Proteins from Thermophilic Microorganisms Structure and Function

LIST OF PARTICIPANTS 11 THERMOPHILIC HYDROLYTIC ENZYMES (PROTEINASES, AMYLASES) 17 Thermal Stability of Homologous Neutral Metalloendopeptidases in Thermo philic and Mesophilic Bacteria: Structural Considerations M. K. PANGBURN, P. L. LEVY, K. A. WALSH and H. NEURATH 19 The Structure and Stability of Thermolysin L. H. WEAVER, W. R. KESTER, K. F. TEN EYCK and B. W. MATTHEWS 31 Studies of the Inhibition of Thermolysin J. FEDER, N. AUFDERHEIDE and B. S. WILDI 41 Effect of EDTA on the Conformational Stability of Thermolysin A. FONTANA, E. BOCCU and F. M. VERONESE 55 Role of a Sulfhydryl Group in the Structure and Function of Alkaline Proteases from a Thermophilic Actinomycete K. MIZUSAWA and F. YOSHIDA 61 Partial Characterization of a Thermophilic Actinomycete Rennin S. LAXER, A. PINSKY and B. BARTOOV 67 Amylase Activity and Stability at High and Low Temperature Depending on Calcium and other Divalent Cations W. HEINEN and A. M. LAUWERS 77 Role of Calcium Ion in the Thermostability of a-Amylase Produced from Bacillus stearothermophilus K. YUTAN I 91 THERMOPHILIC DEHYDROGENASES 105 Thermophilic Glyceraldehyde-3-P Dehydrogenase R. E. AMELUNXEN and R. SINGLETON JR. 107 Glyceraldehyde 3-Phosphate Dehydrogenase from an Extreme Thermophile, Thermus aquaticus 121 J. D. HOCKING and J. I. HARRIS Thermal Properties of Glyceraldehyde 3-Phosphate Dehydrogenase from Escherichia coli A. FONTANA, C. GRANDI, E. BOCCU and F. M. VERONESE 135 Comparative Conformational Properties of Thermophilic and Mesophilic 6-Phosphogluconate Dehydrogenase F. M. VERONESE, C. GRANDI, E. BOCCU and A.

Contenu
Thermophilic Hydrolytic Enzymes (Proteinases, Amylases).- Thermal Stability of Homologous Neutral Metalloendopeptidases in Thermophilic and Mesophilic Bacteria: Structural Considerations.- The Structure and Stability of Thermolysin.- Studies of the Inhibition of Thermolysin.- Effect of EDTA on the Conformational Stability of Thermolysin.- Role of a Sulfhydryl Group in the Structure and Function of Alkaline Proteases from a Thermophilic Actinomycete.- Partial Characterization of a Thermophilic Actinomycete Rennin.- Amylase Activity and Stability at High and Low Temperature Depending on Calcium and other Divalent Cations.- Role of Calcium Ion in the Thermostability of ?-Amylase Produced from Bacillus stearothermophilus.- Thermophilic Dehydrogenases.- Thermophilic Glyceraldehyde-3-P Dehydrogenase.- Glyceraldehyde 3-Phosphate Dehydrogenase from an Extreme Thermophile, Thermus aquaticus.- Thermal Properties of Glyceraldehyde 3-Phosphate Dehydrogenase from Escherichia coli.- Comparative Conformational Properties of Thermophilic and Mesophilic 6-Phosphogluconate Dehydrogenase.- Purification and Properties of Malate Dehydrogenase from Thermus aquaticus.- Purification and some Properties of NADP+-Specific Isocitrate Dehydrogenase from an Extreme Thermophile, Thermus flavus AT-62.- Other Types of Thermophilic Enzymes (Proteins).- Maintainance of Specificity, Information, and Thermostability in Thermophilic Bacillus sp. Glutamine Synthetase.- Phosphofructokinase from Thermophilic Microorganisms.- Purification and Catalytic Properties of Thermostable Fumarase from Bacillus stearothermophilus NU-10 and Thermus X-l.- Analysis of the Thermostability of Enolases.- Isolation and some Properties of Enolase from Bacillus stearothermophilus.- Properties of Enzymes from ClostridiumThermoaceticum and Clostridium Formicoaceticum.- Chemical, Physical and Enzymatic Comparisons of Formyltetrahydro-folate Synthetases from Thermo- and Mesophilic Clostridia.- Anaplerotic Enzymes of Acetate and Pyruvate Metabolism: Distinctive Characteristics in Bacillus stearothermophilus.- Oxygen Induced Transformation of the NADH-Nitrate Oxidoreductase from Bacillus stearothermophilus.- Thermophilic Phycocyanin.- Ferredoxin from Bacillus stearothermophilus.- Structural and Metabolic Basis of Thermophily (Protein synthesis system, Nucleic acids, Membranes, Temperature-adaptation, Genetics, etc. ).- Biochemical Studies of an Extreme Thermophile Thermus thermophilus: Thermal Stabilities of Cell Constituents and a Bacteriophage.- The Effect of Polyamines on the Thermostability of a Cell Free Protein Synthesizing System of an Extreme Thermophile.- Purification, Characterization and Mechanism of Action of Several Aminoacyl-tRNA Synthetases from Bacillus stearothermophilus.- Thermophilicity and Thermostability of RNA Polymerases from Two Strains of Caldariella acidophila, an Extremely Thermo-acidophi-lic Bacterium.- Thermophilic and Mesophilic Enzymes from Bacillus caldotenax and Bacillus stearothermophilus: Properties, Relationships and Formation.- Studies on the Genetical and Biochemical Basis of Thermophily.- Bacterial Diversity at High Temperature.- Round Table and General Discussion on Structure and Function of Thermophilic Enzymes and Proteins.- Author Index.