Soft Probes for Bio-electrochemical Imaging

This book discusses the development of various reliable scanning electrochemical microscopy (SECM) imaging techniques for studying the distribution of biomarkers and nanomaterials in thin and thick animal samples, plant antioxidant (AO) defense systems, as well as human melanoma. The authors demonstrate that SECM could improve the diagnosis and understanding of different melanoma stages on the basis of highly resolved maps of the tyrosinase distribution. Tyrosinase is the key enzyme involved in fruit maturation and is a biomarker for melanoma. As such the book presents various tyrosinase SECM detection strategies developed for the analysis of the spatial distribution of tyrosinase in melanoma and in banana samples. It describes the first imaging of the redox active proteins within the entire mouse heart with an SECM system using a spider probe composed of eight independent microelectrodes. Further, it investigates distributions of injected graphene nanoribbons (GONRs) for drug deliveryby Soft-Probe-SECM. Lastly, the book outlines a non-invasive electrochemical strategy for mapping the AO activity of apple peel using Soft-Probe-SECM.

Nominated as an outstanding Ph.D. thesis by the École Polytechnique Fédérale de Lausanne, Switzerland Presents pioneer work on electrochemical imaging using soft probe scanning in contact mode Provides a new perspective on bio-imaging that differs from all the conventional imaging techniques

Auteur
Dr. Tzu-En Lin is currently a postdoctoral researcher with a specialization in electrochemistry at École Polytechnique Fédérale de Lausanne, Switzerland (EPFL). She obtained her PhD degree from EPFL and her master's degree from National Taiwan University. Her supervisor is Prof. Hubert Girault, an expert in physical and analytical chemistry, battery technology and hydrogen car development. She was admitted to the US Stanford University-EPFL exchange program as a visiting scholar. Her work on banana and skin cancer has been reported worldwide.

Contenu
Introduction.- Experimental Part.- Multiple SECM Mapping of Tyrosinase in Micro-Contact Printed Fruit Samples on Polyvinylidene Fluoride Membrane.- Monitoring Tyrosinase Expression in Non-Metastatic and Metastatic Melanoma Tissues by SECM.- From Tissues to Organs: Soft Electrochemical Probes for Scanning Human and Animal Tissues.- Imaging the Distribution of Graphene Oxide Nanoribbons in Mice Livers by Soft Probe SECM.- Mapping the Antioxidant Activity of Apple Peels with Soft Probe Scanning Electrochemical Microscopy.- General Conclusions and Future Perspectives.