Pattern Formation in Zebrafish

Vertebrate embryos develop from a single cell via a complex succession of cell divisions, movements and inductive interactions. The zebrafish, Danio rerio, emerged in recent years as an excellent system in which to study genetic underpinnings of normal human development and its pathologies. Large-scale genetic screens identified thousands of mutant variants that allow in vivo dissection of developmental processes at single cell and molecular resolution. This book provides the first comprehensive overview of zebrafish embryogenesis: formation and patterning of germ layers, gastrulation movements, and aspects of organogenesis, including formation of somites, cardiovascular system, pronephros and eyes.

What makes an ideal model system to study embryonic development? The answer to this question has varied in the course of scientific quest, depending on the available experimental approaches. Yet, at the beginning of the new millennium, most of the mainstream model organisms have one feature in common, they are amenable to genetic analysis. Indeed, the genetic approach has proven remarkably effective in the dissection of complex biological phenomena, starting with the regulation of the lactose operon in Escherichia coli, the decision between the lysogenic and lytic cycles of the lambda phage and the yeast cell cycle control. The genetic screens for mutations affecting embryonic development of a fruit fly, Drosophila melanogaster, carried out by Christiane Niisslein-Volhard, Eric Wieschaus and Edward Lewis, marked the turning point in experimental approaches to embryonic development of more complex organisms. The resulting mutants facilitated identification of the key regulatory molecules, while their phenotypic classes hinted at the underlying regulatory mechanisms. This work not only provided a paradigm for genetic dissection of other developmental processes, but also inspired many budding embryologists throughout the world. Soon thereafter, George Streisinger noted that, among vertebrates, the zebrafish, Danio rerio, offered many features facilitating the dissection of nervous system development and function. Among these, transparency of the embryo, ease of husbandry, and the highly prolific nature make the zebrafish a good genetic model system.

The first book that comprehensively describes the mechanisms of early patterning and morphogenesis in zebrafish Summarizes the current knowledge and the key questions for the next decade of reseach All authors are leading experts in the field Includes supplementary material: sn.pub/extras

Contenu
I. Germ Layer Formation and Early Patterning.- Formation and Patterning Roles of the Yolk Syncytial Layer.- Mesoderm Induction and Patterning.- The Guts of Endoderm Formation.- Organizer Formation and Function.- Dorsoventral Patterning in the Zebrafish: Bone Morphogenetic Proteins and Beyond.- Specification of Left-Right Asymmetry.- II. Gastrulation Movements.- Life at the Edge: Epiboly and Involution in the Zebrafish.- Cellular and Genetic Mechanisms of Convergence and Extension.- Primordial Germ Cell Development in Zebrafish.- III. Neural Development.- Patterning the Zebrafish Central Nervous System.- Specification of the Zebrafish Neural Crest.- Neurogenesis and Specification of Neuronal Identity.- Cellular, Genetic and Molecular Mechanisms of Axon Guidance in the Zebrafish.- IV. Aspects of Organogenesis.- Somitogenesis.- Cardiovascular System.- The Pronephros.- The Zebrafish Eye: Developmental and Genetic Analysis.- References.