Methods in Biological Oxidative Stress

Oxidative damage appears to play a central role in the development of a wide range of tissue pathology, including neurodegenerative disease, drug side-effects, xenobiotic toxicity, carcinogenesis, and the aging process, to name just a few. Because of the centrality of oxidative processes to normal and abnormal tissue function, it has become imperative to develop appropriate analytical techniques to facilitate the quantitation of significant reactants. Without advances in methodology, corresponding advances in our knowledge of underlying biochemical events will be necessarily limited. Drs. Hensley and Floyd have done an outstanding job of assembling the work of world-class experts into Methods in Biological Oxidative Stress. The contributors have presented concise, yet thorough, descriptions of the state-of-the-art methods that any investigator working in the field needs to access. Mannfred A. Hollinger v Preface Free radicals and reactive oxidizing agents were once ignored as biochemical entities not worth close scrutiny, but are now recognized as causes or contributing factors in dozens, if not hundreds, of disease states. In addition, free radical metabolisms of xenobiotics have become increasingly important to pharmacologists. Accordingly, the need has arisen to accurately quantify reactive oxygen species and their byproducts. Methods in Biological Oxidative Stress is practical in scope, providing the details of up-to-date techniques for measuring oxidative stress and detecting oxidizing agents both in vitro and in vivo. The contributors are recognized experts in the field of oxidative stress who have developed novel strategies for studying biological oxidations.

Includes supplementary material: sn.pub/extras

Texte du rabat
Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method.
Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry.

Contenu
Lipids.- Measurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array Detection.- Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC with Fluorescence Detection.- Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization.- Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization.- Measurement of F4-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization.- Immunoassays for Lipid Peroxidation End Products.- Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological Matrices.- HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human Plasma.- DNA, Protein, and Amino Acids.- Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein Oxidation.- Gas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids.- Isotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and Tissues.- Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis.- Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe.- Analysis of Neuroketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry.- Measurement of Isoketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass Spectrometry.- Bioassay of 2?-Deoxyguanosine/8-Hydroxy-2?-Deoxyguanosine by HPLC With Electrochemical/Photodiode Array Detection.- HPLC With ElectrochemicalDetection Analysis of 3-Nitrotyrosine in Human Plasma.- Reactive Oxygen Species and Reactive Nitrogen Species.- Protein Carbonyl LevelsAn Assessment of Protein Oxidation.- Fluorogenic Analysis of H2O2 in Biological Materials.- Detection of Reactive Oxygen Species by Flow Cytometry.- Nitrite Determination by Colorimetric and Fluorometric Griess Diazotization Assays.- Protein Carbonyl Determination Using Biotin Hydrazide.- Real-Time, In-Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary Flow.