Carbohydrate-Based Interactions at the Molecular and the Cellular Level

This book offers a clearly written and highly accessible account of two different aspects of carbohydrate chemistry. Carbohydrates are an essential component of life and have many important biological functions, but the details of how carbohydrates interact with other biomolecules to mediate biological signalling remain unclear. Firstly, this thesis details innovative methods to mine protein structural data to uncover new features of carbohydrate-based interactions. It also explains these findings using physical chemistry, specifically CHpi interactions associated with the properties of the interacting partners.

Carbohydrates are also critical for tissue growth and development, yet are underexploited in the materials science that underpins much of regenerative medicine. As such, the second part of this thesis describes a diverse array of techniques ranging from synthetic chemistry and enzymatic synthesis to prepare a wide variety of carbohydrates, and materials chemistry to prepare glycosylated hydrogels, to cell biology to determine the effects on cellular development for tissue engineering applications.

Nominated as an outstanding Ph.D. thesis by the University of Bristol, UK Presents an in-depth analysis of carbohydrate binding by proteins Offers new insights into non-covalent interactions in chemistry and biology Describes new methods to direct cellular development

Auteur
Dr Kieran Hudson received a Master of Chemistry degree from the University of Oxford, UK in 2011. During his research there he developed an interest in the intersection of chemistry and biology, particularly in how small structural differences in biomolecules have substantial effects in nature. He developed this interest during his postgraduate research at the University of Bristol, UK, from where he obtained a PhD with a Faculty of Science Commendation in 2016. Dr Hudson is currently continuing his research as a postdoctoral fellow at the University of British Columbia, Canada.

Contenu
Introduction.- Protein-Carbohydrate Interactions in Protein X-ray Crystal Structures.- The Nature of Protein-Carbohydrate Interactions.- The Modifiable Scaffold for Tissue Engineering.- Carbohydrate Modifiers for Tissue Engineering Scaffolds.- Conclusions and Future Directions.- Experimental Details and Supplementary Data.