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Informationen zum Autor Dr Anne Bridgen , previously of The University of Ulster is a molecular virologist with extensive research and teaching experience. She was the first scientist to recover infectious virus particles from DNA clones of a segmented RNA virus. Dr Bridgen knows the field and its main players well and has both the knowledge and experience to bring individual expert contributions together around the common theme of reverse genetics. She is currently providing consultancy for a BBSRC grant based at IAH Pirbright. Klappentext Reverse genetics, the manipulation of the DNA of RNA viruses to create a modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells over the past fourteen years. Since most severe viral human and animal pathogens are RNA viruses, including those responsible for polio, hepatitis and influenza, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases.Written by a team of international experts in the field, this book provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. From influenza A virus vaccines, to fluorescent measles virus and quasispecies, this guide explores the progress researchers have made in our current understanding of viral disease and considers where possible applications may lead in the future. Zusammenfassung Reverse genetics, the manipulation of the DNA of RNA viruses to create a modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells over the past fourteen years. Since most severe viral human and animal pathogens are RNA viruses, including those responsible for polio, hepatitis and influenza, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases.Written by a team of international experts in the field, this book provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. From influenza A virus vaccines, to fluorescent measles virus and quasispecies, this guide explores the progress researchers have made in our current understanding of viral disease and considers where possible applications may lead in the future. Inhaltsverzeichnis List of contributors xiAcknowledgements xiii1 Introduction 1Anne Bridgen1.1 Background 11.2 Reverse genetics for different classes of genome 21.3 Methodology 51.4 Difficulties in establishing a reverse genetics system 111.5 Recent developments 131.6 Are there any boundaries for conducting reverse genetics? 13References 15Part I Positive sense RNA viruses 252 Coronavirus reverse genetics 27Maria Armesto, Kirsten Bentley, Erica Bickerton, Sarah Keep and Paul Britton2.1 The Coronavirinae 272.2 Infectious bronchitis 282.3 Coronavirus genome organisation 292.4 The coronavirus replication cycle 302.5 Development of reverse genetics system for coronaviruses including IBV 332.6 Reverse genetics system for IBV 372.7 Reverse genetics systems for the modification of coronavirus genomes 402.8 Using coronavirus reverse genetics systems for gene delivery 49Acknowledgements 51References 513 Reverse genetic tools to study hepatitis C virus 64Alexander Ploss3.1 Introduction: hepatitis C 643.2 Hepatitis C virus 653.3 Construction of infectious clones for hepatitis C virus 683.4 Study of HCV RNA replication in cell culture systems 683.5 Use of HCV...
Auteur
Dr Anne Bridgen, previously of The University of Ulster is a molecular virologist with extensive research and teaching experience. She was the first scientist to recover infectious virus particles from DNA clones of a segmented RNA virus. Dr Bridgen knows the field and its main players well and has both the knowledge and experience to bring individual expert contributions together around the common theme of reverse genetics.
She is currently providing consultancy for a BBSRC grant based at IAH Pirbright.
Texte du rabat
Reverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases. Reverse Genetics of RNA Viruses provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field. An authoritative, comprehensive overview of reverse genetics in RNA Viruses. Includes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. * Written by a team of international experts, including some of the leading researchers in the field.
Résumé
Reverse genetics, the manipulation of the DNA of RNA viruses to create a modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells over the past fourteen years. Since most severe viral human and animal pathogens are RNA viruses, including those responsible for polio, hepatitis and influenza, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases. Written by a team of international experts in the field, this book provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. From influenza A virus vaccines, to fluorescent measles virus and quasispecies, this guide explores the progress researchers have made in our current understanding of viral disease and considers where possible applications may lead in the future.
Contenu
List of contributors xi Acknowledgements xiii 1 Introduction 1 Anne Bridgen 1.1 Background 1 1.2 Reverse genetics for different classes of genome 2 1.3 Methodology 5 1.4 Difficulties in establishing a reverse genetics system 11 1.5 Recent developments 13 1.6 Are there any boundaries for conducting reverse genetics? 13 References 15 Part I Positive sense RNA viruses 25 2 Coronavirus reverse genetics 27 Maria Armesto, Kirsten Bentley, Erica Bickerton, Sarah Keep and Paul Britton 2.1 The Coronavirinae 27 2.2 Infectious bronchitis 28 2.3 Coronavirus genome organisation 29 2.4 The coronavirus replication cycle 30 2.5 Development of reverse genetics system for coronaviruses including IBV 33 2.6 Reverse genetics system for IBV 37 2.7 Reverse genetics systems for the modification of coronavirus genomes 40 2.8 Using coronavirus reverse genetics systems for gene delivery 49 Acknowledgements 51 References…