Zebrafish

This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. Zebrafish: Methods and Protocols, Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish, applications of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection and host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuits and their role in generating behavior. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a useful complement to the first book for new and experienced zebrafish researcher alike.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Chemical Screening in Zebrafish.- TALEN-Mediated Mutagenesis and Genome Editing.- Homology Independent Integration of Plasmid DNA into the Zebrafish Genome.- Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System.- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish.- Testing of cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish using PhiC31 Integrase.- Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP Positive Cells from Transgenic Zebrafish Lines.- Construction of the Inbred Strain.- Zebrafish as a Model for the Study of Solid Malignancies.- Melanoma Regression and Recurrence in Zebrafish.- Imaging of Human Cancer Cell Proliferation, Invasion and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model.- Modeling Leukemogenesis in the Zebrafish using Genetic and Xenograft Models.- Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos.- Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.- Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.- Studying Lipid Metabolism and Transport During Zebrafish Development.- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish.- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.- Quantifying Aggressive Behaviour in Zebrafish.- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.- A Practical Guide to Light Sheet Microscopy.- Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish.- Fiber Optic-Based Photostimulation of Larval Zebrafish.- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.