Yeast-based functional genomics and proteomic technologies have increased our understanding of bacterial, yeast, fly, worm and human gene functions. In this book, experts in the field offer stand-alone protocols suitable for daily use in research laboratories.

Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology™ series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.

Covers a wide range of state-of-the-art yeast-based functional genomics and proteomics technologies

Provides an easily accessible reference volume for most important yeast-based functional genomics and proteomics technologies written by leading researchers in the field

Comprehensive guide to running and analyzing yeast-based assays in functional genomics and proteomics

Includes supplementary material: sn.pub/extras

Klappentext

Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology™ series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.

Inhalt

1. Comparative Genome Hybridization on Tiling Microarrays to Detect Aneuplodies in Yeast Barry Dion and Grant W. Brown 2. Identification of Transcription Factor Targets by Phenotypic Activation and Microarray Expression Profiling in Yeast Gordon Chua 3. SGAM, an Array-based Approach for High-Resolution Genetic Mapping in Saccharomyces cerevisiae Michael Costanzo and Charles Boone 4. Reporter-based Synthetic Genetic Array Analysis: A Functional Genomics Approach for Investigating the Cell Cycle in Saccharomyces cerevisiae Holly Elizabeth Sassi, Nazareth Bastajian, Pinay Kainth, and Brenda Jean Andrews 5. The Fidgety Yeast: Focus on High-Resolution Live Yeast Cell Microscopy Heimo Wolinski, Klaus Natter, and Sepp D. Kohlwein 6. A Genomic Approach to Yeast Chronological Aging Christopher R. Burtner, Christopher J. Murakami, and Matt Kaeberlein 7. Chemogenomic Approaches to Elucidation of Gene Function and Genetic Pathways Sarah E. Pierce, Ron W. Davis, Corey Nislow, and Guri Giaever 8. Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens Benjamin Newcomb and Antonio Bedalov 9. Exploiting Yeast Genetics to Inform Therapeutic Strategies for Huntington's Disease Flaviano Giorgini and Paul J. Muchowski 10. Global Proteomic Analysis of S. cerevisiae (GPS) Identifies Molecular Pathways of Histone Modifications Jessica Jackson and Ali Shilatifard 11. Systematic Characterization of the Protein Interaction Network and Protein Complexes in Saccharomyces cerevisiae Using Tandem Affinity Purification (TAP) and Mass Spectrometry Mohan Babu, Nevan J. Krogan, Donald E. Awrey, Andrew Emili, and Jack F. Greenblatt 12. Protein Microarrays Joseph Fasolo and MichaelSnyder 13. Analysis of Protein-Protein Interactions Using Array-Based Yeast Two-Hybrid Screens Seesandra V. Rajagopal and Peter Uetz 14. Analysis of Membrane Protein Complexes Using the Split-Ubiquitin Membrane Yeast Two-Hybrid (MYTH) System Saranya Kittanakom, Matthew Chuk, Jamie Snider, Victoria Wong, Dawn Edmonds, Apostolos Lydakis, Zhaolei Zhang, Daniel Auerbach, and Igor Stagljar 15. Computational Analysis of the Yeast Proteome: Understanding and Exploiting Functional Specificity in Genomic Data Curtis Huttenhower, Chad L. Myers, Matthew A. Hibbs, and Olga G. Troyanskaya