This detailed volume explores the condition of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and the recent techniques used to investigate the dysfunctional pathophysiology in patients. Beginning with a section on diagnosis, the book continues by covering the chronic cellular and molecular changes in the peripheral immune system that affect their molecular homeostasis, metabolic changes, as well as imaging technologies to understand the brain's dysfunction in aberrantly regulating body physiology in ME/CFS. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): Methods and Protocols serves as an ideal guide for clinicians and researchers working to better understand post-viral/stressor conditions in order to improve the lives of those affected with ME/CFS and, most recently, long COVID.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

How a Clinician Makes a Diagnosis for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).- Using the Ratio of Phosphorylated to Non-Phosphorylated Forms of Stress Kinase PKR as a Potential Diagnostic Test for ME/CFS.- Using Single-Cell Raman Microspectroscopy to Profile Human Peripheral Blood Mononuclear Cells.- MicroRNA Profiling of Blood Extracellular Vesicles in ME/CFS.- Deep Immunophenotyping in ME/CFS Using Spectral Cytometry.- Analysis of Transient Receptor Potential Ion Channels in ME/CFS.- Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis.- Quantitative Proteomics on Immune Cells of ME/CFS Patients Using SWATH-MS.- Application of DNA Methylome Analysis to Patients with ME/CFS.- Repeated Cardiopulmonary Exercise Testing of ME/CFS Patients.- Real-Time Measurement of Mitochondrial Function and Glycolysis in Lymphoblastoid Cell Lines.- Mitochondrial Measures in Primary Cells Isolated from Patients with ME/CFS.- Measuring Biomarkers of Oxidative Stress in ME/CFS Patients.- Measurement of Genetic Variations in ME/CFS Patients in the IDO2 Gene Encoding an Enzyme Metabolizing Tryptophan.- Review of Neuroimaging Methods in ME/CFS.- Profiling Antibody Reactivity to Gut Microbes in ME/CFS Patients.