This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
irus-induced Gene Silencing in Lilies using Cucumber Mosaic Virus Vectors.- Virus-Induced Gene Silencing in Poaceae Using a Foxtail Mosaic Virus Vector.- Virus-Induced Gene Silencing (VIGS) in Chili Pepper ( Capsicum spp.).- Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species.- Virus Induced Gene Silencing (VIGS) in Cassava using Geminivirus Agroclones.- Virus Induced Gene Silencing of Cell Wall Genes in Flax ( Linum usitatissimum ).- Virus-Induced Gene Silencing to Investigate Alkaloid Biosynthesis in Opium Poppy.- A Biolistic-mediated Virus-Induced Gene Silencing in Apocynaceae to Map Biosynthetic Pathways of Alkaloids.- Virus Induced Gene Silencing in Nepeta.- Virus Induced Gene Silencing in Sweet basil ( Ocimum basilicum ).- Virus-Induced Gene Silencing for Gene Function Studies in Withania somnifera , an Important Indian Medicinal Plant.- A Prunus necrotic ringspot virus (PNRSV)-based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees.- Virus-Induced Gene Silencing in Olive Tree (Oleaceae).- ALSV-based Virus-Induced Gene Silencing in Apple Tree ( Malus x domestica L. ).- Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia.- Virus-induced Gene Silencing in Rose Flowers.- Virus-induced Gene Silencing (VIGS) in Flax ( Linum usitatissimum L.) Seedcoat: Description of an Effective Procedure using the transparent testa 2 Gene as a Selectable Marker.- Virus-Based microRNA Silencing in Plants.