Vascular Morphogenesis: Methods and Protocols provides a range of techniques for studying vascular morphogenesis in vivo and in vitro, reflecting advances in the field. Endothelial cell signaling is currently believed to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation and postnatal tissue remodeling. Understanding the concept of vascular bed specificity represents a major challenge for future investigations. Indeed, one of the most interesting theoretical perspectives and practical applications of endothelial cell signaling is the possibility for these cells to maintain their inductive potential during adult life. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative, Vascular Morphogenesis: Methods and Protocols will serve as a instructive and useful reference for cell biologists, anatomists, pathologists and physiologists, as well as all those interested in this area of biological study.

Includes cutting-edge methods and protocols in vascular morphogenesis research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

The Development of the Vascular System: An Historical Overview.- The Corneal Pocket Assay.- The Mouse Hindbrain: An In Vivo Model to Analyze Developmental Angiogenesis.- Three Dimensional In Vitro Assay of Endothelial Cell Invasion and Capillary Tube Morphogenesis.- Vascular Casting for the Study of Vascular Morphogenesis.- Cell-Based Computational Modeling of Vascular Morphogenesis Using Tissue Simulation Toolkit.- miRNAs Expression Profile in Zebrafish Developing Vessels.- Imaging of Cardiovascular Development in Mammalian Embryos Using Optical Coherence Tomography.- Live Confocal Microscopy of the Developing Mouse Embryonic Yolk Sac Vasculature.- Zebrafish Embryo Intersegmental Vessels: A Tool for Investigating Sprouting Angiogenesis.- Avian Area Vasculosa and CAM as Rapid In Vivo Pro- and Anti-Angiogenic Models.- Investigating In Vitro Angiogenesis by Computer-Assisted Image Analysis and Computational Simulation.- A Chimeric Embryoid Body Model to Study Vascular Morphogenesis.- Avians as a Model System of Vascular Development.- Microfluidic Model of Angiogenic Sprouting.- The Rat Aortic Ring Model of Angiogenesis.- The Co-Culture Organotypic Assay of Angiogenesis.