Transfer of Cell Constituents into Eukaryotic Cells

This book is a record of the proceedings of a NATO Advanced Study Institute on "Transfer of Cell Constituents into Eukaryotic Cells" held September 2-14, 1979, at Sintra-Estoril, Portugal. Transfer of cell constituents into living cells can be brought about by a variety of ways, namely by microinjection with micropi pettes (small somatic cells, oocytes and plant cells), by microin jection using fusion methods (red cell mediated microinjection, li posome mediated transfer, transfer of membrane components, cell hy bridization and fusion of cell fragments)and by means of uptake (DNA and chromosome gene transfer). Prompted by the development~ in this exciting and expanding field of cell biology it seemed timely to meet to review the exist ing knowledge and to speculate on future research applications. The lectures published here reflect the diversity of research currently underway using these techniques. The final outcome of the meeting was positive as it promoted collaboration between laboratories and, most important, created strong scientific and social links between individuals. We wish to express our appreciation to Dr. Maria C. Lechner and Mrs. Helena Gata for providing valuable advice and help through the initial planning stages of this meeting. Finally, we are indebted to Ms. Lisbeth Heilesen for her outstanding organization and admi- v vi PREFACE stration of the meeting and for typing all the manuscripts of this book. The meeting was held under the sponsorship of NATO with partial support from the Gulbenkian Foundation.

Inhalt
Microinjection of Somatic Cells with Micropipettes.- 1. Microinjection of Somatic Cells with Micropipettes and PEG-Erythrocyte Ghost Mediated Microinjection.- 2. Microinjection of Cellular and Viral mRNAs.- 3. Expression of Rabbit Globin mRNA Injected into Fused HeLa Cells.- 4. Biological Activity of Simian Virus 40 DNA Fragments and T-Antigen Tested by Microinjection into Tissue Culture Cells.- 5. Aspects of Cell Architecture and Locomotion.- Ghost Mediated Microinjection and Transfer of Membrane Components.- 6. Red Cell-Mediated Microinjection Studies on Protein Degradation.- 7. Transfer of Functional Compoments into Plasma Membrane of Living Cells: A New Tool in Membrane Research.- Liposome Mediated Transfer.- 8. Liposomes as Macromolecular Carriers for the Introduction of RNA and DNA into Cells.- 9. Liposomes in Drug Targeting.- Fusion of Cell Fragments and Cell Hybrids.- 10. Fusion of Cell Fragments as a Method in Cell Genetics.- 11. B-Cell and Epstein-Barr Virus (EB) Associated Functions in Human Cells and Hybrids.- 12. Tumorigenicity, Actin Cables and Gene Expression in Mouse CLID × CHO Cell Hybrids.- DNA and Chromosome Mediated Gene Transfer.- 13. Gene Transfer in Eukaryotes.- 14. DNA Mediated Gene Transfer between Mammalian Cells.- 15. Transfer of DNA into Plant Cells with the Ti-Plasmid as a Vector.- Microinjection of Xenopus Oocytes.- 16. Expression of Messenger RNAs Injected into Xenopus Laevis Oocytes.- 17. Surrogate Genetics in the Frog Oocyte.- Transfer of Cell Constituents Into Plant Cells.- 18. Grafts and Transfer of Cell Constituents into the Giant Unicellular Alga Acetabularia.- Contributors.