Thyroid Hormone Nuclear Receptor

This volume aims to detail the advances in the field of Thyroid hormones (THs), namely T3 and T4, and their nuclear receptor TRs from a conceptual and methodological point of view. Chapters guide readers through a general introduction, TR's engineered mice, human genetics of TRs, control of the hypothalamus-pituitary-thyroid (HPT) axis, the relevance of the local THs production and metabolism and pathological consequences of TH/TR alterations. Non-mammalian models are also presented and discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Thyroid Hormone Nuclear Receptor: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Thyroid Hormone Receptors: Several Players for One Hormone and Multiple Functions.- Two Protocols to Study the Interactions of Thyroid Hormone Receptors with Other Proteins and Chromatin.- Expression Analysis of Genes Regulated by Thyroid Hormone in Neural Cells.- In vitro Approaches to Identify Thyroid Hormone Receptor-dependent Transcriptional Response.- Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Phosphorylation.- Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Sumoylation.- Demonstration of the Receptor Site for Thyroid Hormone on Integrin v3.- The Deiodinase Trio and Thyroid Hormone Signaling.- Thyroid Hormones and Derivatives Endogenous Thyroid Hormones and their Targets.- Protocols for Studying how Thyroid Hormone Receptors Trigger Adaptive Thermogenesis in Mice.- Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells in vitro .- Analysis of Physiological Responses to Thyroid Hormones and their Receptors in Bone.- Evaluating the Hypothalamic-Pituitary-Thyroid (HPT) Axis in Mice.- Thyroid Hormone Action: The p43 Mitochondrial Pathway.- Evaluating thyroid Disrupting Chemicals in vivo using Xenopus laevis .- Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples.- Analysis of Thyroid Tumorigenesis in Xenograft Mouse Model.- Human Genetics Of Thyroid Hormone Receptor Beta: Resistance to thyroid Hormone Beta.- TRa Mutations in Human.- Role of Thyroid Hormone Receptor in Amphibian Development.- de novo Transcriptomic Approach To Study Thyroid Hormone Receptor Action in Non-mammalian Models.- Role of TRs in Zebrafish Development.