Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo

With concise coverage of light- and electron-based microscopy, including new cryoelectron techniques, this readily consultable guide allows researchers to make informed decisions on which technique to use, and shows them how to interpret the resulting imagery.

As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.

A concise primer to a variety of both light and electron microscopy techniques allows the reader to make an informed decision regarding which one will be best for their own research An expansive introduction to the analysis of electron microscopy images prepares the reader for a variety of structure determination procedures Each chapter starts with an overview of the material covered Attractive layout with hyperlinks to related sections, figures and references creates a format ideal for quick and easy referencing Full methods section explains how to perform each technique covered Comprehensive explanations and over 80 illustrations lead the reader through the results presented Includes supplementary material: sn.pub/extras

Autorentext
Thom Sharp completed his bachelor's degree in Biochemistry at the University of Bristol, with a year spent a year working at AstraZeneca. He continued in Bristol for his PhD in bionanotechnology, which was performed under the supervision of Dek Woolfson, a protein design chemist, and Paul Verkade, an electron microscopist. Thom is currently at the University of Oxford, where he is developing DNA-templated protein arrays to increase the throughput of single-particle analysis.

Inhalt
Biomolecules & Microscopy.- Materials and Methods.- Experimental Methods.- Imaging Methods.- Experimental Results.- Towards an Atomistic Structure of a De Novo Designed Peptide Fibre.- Developing Novel Probes for Correlative Light Electron Microscopy.