The Tumor Microenvironment

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various omics-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

Includes cutting-edge methods and protocols for tumor microenvironment research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible.- Laser Capture Microdissection as a Tool to Study Tumor Stroma.- Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging.- Studies on the Tumor Vasculature and Coagulant Microenvironment.- A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment.- Measuring Vascular Permeability In Vivo.- Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL).- Analyzing the Tumor Microenvironment by Flow Cytometry.- Detecting Secreted Analytes from Immune Cells: An Overview of Technologies.- Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting.- Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.- Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies.- Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer.- Analysis of Extracellular Vesicles in the Tumor Microenvironment.- Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disc Confocal Microscopy.- Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice.- Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer.- Modeling Breast Tumor Development with a Humanized Mouse Model.- CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice.- Metabolomics Analyses of Cancer Cells in Controlled Microenvironments.- Analysis of the Tumor Microenvironment Transcriptome Via NanoString mRNA and miRNA Expression Profiling.- RNA-Seq as a Tool to Study the Tumor Microenvironment.- Sample Preparation for MassSpectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues.