This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
High-Voltage Paper Electrophoresis (HVPE).- Carbohydrate Gel Electrophoresis.- Capillary Electrophoresis with Detection by Laser-Induced Fluorescence.- Theory and Practice in Measuring In Vitro Extensibility of Growing Plant Cell Walls.- Formation of Cellulose-Based Composites with Hemicelluloses and Pectins using Komagataeibacter Fermentation.- Plant Tissue Cultures.- Protoplast Isolation and Manipulation in the Unicellular Model Plant, Penium margaritaceum.- Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens.- Expression of Cell-Wall Modifying Enzymes in Aspen for Improved Lignocellulose Processing.- Activity and Action of Cell-Wall Transglycanases.- Screening for Cellulolytic Plant Enzymes using Colorimetric and Fluorescence Methods.- Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls.- High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron Microscopy.- Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM).- Raman Imaging of Plant Cell Walls.- Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy.- Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis Seedlings.- Analysis of Plant Cell Walls using High-Throughput Profiling Techniques with Multivariate Methods.- Vibratome Sectioning of Plant Materials for (Immuno)Cytochemical Staining.- Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.- Electron Tomography and Immunogold Labeling as Tools to Analyze de novo Assembly of Plant Cell Walls.- Contributions to Arabinogalactan Proteins Analysis.- In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent InSitu Hybridization, FISH.- Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins.- Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins.- Bioinformatic Identification of Plant Hydroxyproline-Rich Glycoproteins.- Bioinformatics Analysis of Plant Cell Wall Evolution.- Automated Glycan Assembly of Plant Cell Wall Oligosaccharides.- Computerized Molecular Modelling of Carbohydrates.