The Molecular Basis of Viral Replication

the discovery of the "splicing" of the gene transcripts, the list would include the whole molecular genetics of the lambda bacteriophage, the notions of "promotor," "repressor," and "integration," the discovery of the reverse flow of genetic information, the very existence of oncogenes, the S'-terminal "cap" struc ture of eukaryotic mRNAs, ... Electronmicroscopy, ultracentrifugation and tissue culture were the landmarks on the way of the young science. During the past few years, however, a major (and not so silent) revolution took place: recombinant DNA technology with all its might entered in our laboratories, and restriction mapping of cloned genomes and sequencing gels have replaced plaque counting and sucrose gradients. The new techniques have made it possible to "dissect" the entire genome of a virus at the molecular level, and studies that would have been dreamt of just in the mid-seventies became the everyday experiments of our days. With new insight into the structure of viral genomes, and a deeper understanding of the mechanisms that regulate their expression, our view of viruses was bound to change: this volume bears witness to this impressive advance.

Klappentext

the discovery of the "splicing" of the gene transcripts, the list would include the whole molecular genetics of the lambda bacteriophage, the notions of "promotor," "repressor," and "integration," the discovery of the reverse flow of genetic information, the very existence of oncogenes, the S'-terminal "cap" struc­ ture of eukaryotic mRNAs, ... Electronmicroscopy, ultracentrifugation and tissue culture were the landmarks on the way of the young science. During the past few years, however, a major (and not so silent) revolution took place: recombinant DNA technology with all its might entered in our laboratories, and restriction mapping of cloned genomes and sequencing gels have replaced plaque counting and sucrose gradients. The new techniques have made it possible to "dissect" the entire genome of a virus at the molecular level, and studies that would have been dreamt of just in the mid-seventies became the everyday experiments of our days. With new insight into the structure of viral genomes, and a deeper understanding of the mechanisms that regulate their expression, our view of viruses was bound to change: this volume bears witness to this impressive advance.

Inhalt
Section I: Viruses at the Molecular Level.- 1: Viruses: An Overview.- 2: Viral Particles at Atomic Resolution.- Section II: The Basic Processes Involved in Viral Replication.- 3: Strategy of Replication of the Viral Genome.- 4: Posttranscriptional Processing (Splicing and 3?-End Formation) of Nuclear Messenger RNA Precursors in Vitro.- 5: Regulation of Translation of Viral mRNAs.- Section III: Interference and Interferon.- 6: Biochemical Aspects of Interferon Action.- 7: The Role of Defective Interfering (DI) Particles in Viral Infection.- Section IV: The Strategies of Replication.- 8: Picornaviruses at the Molecular Level.- 9: The Replication of Togaviridae and Flaviviridae at the Molecular Level.- 10: The Molecular Biology of Coronaviruses.- 11: Molecular Basis of Rhabdovirus Replication.- 12: The Molecular Biology of Paramyxoviruses.- 13: Influenza Viruses: Genome Structure, Transcription and Replication of Viral RNA.- 14: The Molecular Biology of Arenaviruses.- 15: The Reovirus Family at the Molecular Level.- 16: The Molecular Biology of Retroviruses.- 17: The Molecular Biology of Hepatitis B Virus.- 18: The Biology of the Papillomaviruses.- 19: Biological and Molecular Aspects of Simian Virus 40 (SV-40) and Polyomavirus Replication.- 20: The Molecular Biology of Adenoviruses.- 21: The Molecular Biology of Poxviruses.- 22: Herpesviruses: biology, Gene Regulation, Latency, and Genetic Engineering.- Section V: the Maratea Conference.- The Maratea Conference.- List of Participants.