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The Blood-Brain and Other Neural Barriers

  • Kartonierter Einband
  • 516 Seiten
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Opening with components of the neurovascular unit and the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, this book ... Weiterlesen
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Opening with components of the neurovascular unit and the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, this book reviews imaging techniques, molecular study methods, and techniques for delivering therapeutic agents across the BBB.

Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases. In The Blood-Brain and Other Neural Barriers: Reviews and Protocols, experts in the field present a series of cutting-edge protocols which can be used to study the barriers. Opening with detailed information on components of the neurovascular unit as well as the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, the book continues with meticulous techniques to image the barriers in humans and experimental animals, followed by cutting-edge molecular techniques to study the BBB and novel models to study the barriers, and it concludes with techniques for the delivery of therapeutic agents across the BBB. Written in the highly successful Methods in Molecular Biology(TM) series format, protocol chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, The Blood-Brain and Other Neural Barriers: Reviews and Protocols will aid the research efforts of not only graduate students but also more experienced investigators and support future studies of these vital systems.


Part I: Biology of the Barriers

1. Morphology and Properties of Brain Endothelial Cells
Sukriti Nag

2. Morphology and Properties of Pericytes
Paula Dore-Duffy and Kristen Cleary

3. Morphology and Properties of Astrocytes
Sukriti Nag

4. The Blood-Cerebrospinal Fluid Barrier: Structure and Functional Significance
Conrad E. Johanson, Edward G. Stopa, and Paul N. McMillan

5. The Blood-Retinal Barrier: Structure and Functional Significance
E. Aaron Runkle and David A. Antonetti

6. The Blood-Nerve Barrier: Structure and Functional Significance
Ananda Weerasuriya and Andrew P. Mizisin

Part II: Imaging the Barriers

7. Detection of Multiple Proteins in Intracerebral Vessels by Confocal Microscopy
Janet L. Manias, Anish Kapadia, and Sukriti Nag

8. Multiparametric Magnetic Resonance Imaging and Repeated Measurements of Blood-Brain Barrier Permeability to Contrast Agents
Tavarekere N. Nagaraja, Robert A. Knight, James R. Ewing, Kishor Karki, Vijaya Nagesh, and Joseph D. Fenstermacher

9. Detection of Brain Pathology by Magnetic Resonance Imaging of Iron Oxide Microparticles
Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis, and Robin P. Choudhury

10. Measuring the Integrity of the Human Blood-Brain Barrier Using Magnetic Resonance Imaging
Andrea Kassner and Rebecca Thornhill

11. Assessing Blood-Cerebrospinal Fluid Barrier Permeability in the Rat Embryo
Norman R. Saunders, C. Joakim Ek, Mark D. Habgood, Pia Johansson, Shane Liddelow, and Katarzyna M. Dziegielewska

12. Detection of Blood-Nerve Barrier Permeability by Magnetic Resonance Imaging
Carsten Wessig

Part III: Molecular Techniques to Study the Blood-Brain Barrier

13. Isolation of Human Brain Endothelial Cells and Characterization of Lipid Raft-Associated Proteins by Mass Spectroscopy
Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers, and Alexandre Prat

14. Analysis of Mouse Brain Microvascular Endothelium Using Laser Capture Microdissection Coupled with Proteomics
Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan Lu, Shiaw-Lin Wu, William S. Hancock, and Joel S. Pachter

15. Molecular and Functional Characterization of P-Glycoprotein In vitro
Gary N.Y. Chan and Reina Bendayan

16. Methods to Study Glycoproteins at the Blood-Brain Barrier Using Mass Spectrometry
Arsalan S. Haqqani, Jennifer J. Hill, James Mullen, and Danica B. Stanimirovic

Part IV: Models to Study the Barriers

17. Novel Models for Studying the Blood-Brain and Blood-Eye Barriers in Drosophila
Robert L. Pinsonneault, Nasima Mayer, Fahima Mayer, Nebiyu Tegegn, and Roland J. Bainton

18. Zebrafish Model of the Blood-Brain Barrier: Morphological and Permeability Studies
Brian P. Eliceiri, Ana Maria Gonzalez, and Andrew Baird

19. Methods to Assess Pericyte-Endothelial Cell Interactions in a Co-Culture Model
Gokulan Thanabalasundaram, Jehad El-Gindi, Mira Lischper, and Hans-Joachim Galla

20. Isolation and Properties of an In vitro Human Outer Blood-Retinal Barrier Model
Robin D. Hamilton and Lopa Leach

21. Isolation and Properties of Endothelial Cells Forming the Blood-Nerve Barrier
Yasuteru Sano and Takashi Kanda

Part V: Delivery of Therapeutic Agents Across the Barriers

22. Treatment of Focal Brain Ischemia with Viral Vector-Mediated Gene Transfer
Hua Su and Guo-Yuan Yang

23. Blood-Brain Barrier Disruption in the Treatment of Brain Tumors
Marie Blanchette and David Fortin

24. Integrated Platform for Brain Imaging and Drug Delivery Across the Blood-Brain Barrier
Umar Iqbal, Abedelnasser Abulrob, and Danica B. Stanimirovic

25. Targeting the Choroid Plexus-CSF-Brain Nexus Using Peptides Identified by Phage Display
Andrew Baird, Brian P. Eliceiri, Conrad E. Johanson, Edward G. Stopa, Wendy Leadbeater, and Ana Maria Gonzalez


Titel: The Blood-Brain and Other Neural Barriers
Untertitel: Reviews and Protocols
EAN: 9781493961443
ISBN: 1493961446
Format: Kartonierter Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 516
Gewicht: 960g
Größe: H254mm x B178mm x T27mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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