T-Cell Trafficking

Recent discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. This book explores how the development of novel techniques has led to an increased understanding of lymphocyte trafficking.

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols , expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.

Includes detailed description of cutting edge imaging techniques, including multiphoton microscopy, and software-based analysis of T cell infiltration in vivo Describes methods for setting up assays for the observation of lymphocyte migration under flow in vitro Provides description of effective genetic manipulation of the endothelium Includes assays for the investigation of recently described migratory mechanisms, including fugetaxis

Inhalt
Introductory Review.- How T Cells Find Their Way Around.- Migration of T Cells In Vitro.- Live Imaging of LeukocyteEndothelium Interactions.- Leucocyte Adhesion Under Haemodynamic Flow Conditions.- Influence of Stromal Cells on Lymphocyte Adhesion and Migration on Endothelial Cells.- Discriminating Between the Paracellular and Transcellular Routes of Diapedesis.- Monitoring RhoGTPase Activity in Lymphocytes.- Visualisation of Signalling in Immune Cells.- Methods for Quantitation of Leukocyte Chemotaxis and Fugetaxis.- Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes.- Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells.- Migration of T Cells In Vivo.- Visualisation of Lymphoid Organ Development.- Single-Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy.- Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy.- Applying an Adaptive Watershed to the Tissue Cell Quantification During T-Cell Migration and Embryonic Development.- Monitoring T-Cell Migration in Human Diseases.- Identifying Homing Interactions in T-Cell Traffic in Human Disease.- Tracking Antigen-Experienced Effector T Cells In Vitro and In Vivo.- Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo.