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T Cell Protocols

  • Fester Einband
  • 184 Seiten
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Leseprobe
Fully updated and ready to use, this second edition is an ideal guide for young investigators new to the complexities of immunolog... Weiterlesen
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Beschreibung

Fully updated and ready to use, this second edition is an ideal guide for young investigators new to the complexities of immunology, and a valuable resource for experienced scientists wanting clear descriptions of new methods in this vital field of study.

This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes. The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches. Thechoiceofchapterswasmadetakingintoaccounttwopoints:First,manyofthe techniquesthathavebeen usedfor sometimehave beenupgraded duringthe pastfew years given the greater availability of a variety of products (i.e. cytokines, chemokines, monoclonal antibodies), of refined technical devices (i.e. novel cell culture and cell analysis equipments), and the development of novel instrumentation (i.e. multipa- metric flow cytometers, confocal microscopes). Therefore, in several chapters ''old techniques'', which remain fundamental to T cell immunology, are described in their ''modern'' versions. Secondly, the technical advancement has generated the possibility to establish novel assays to investigate T cell physiology. This is reflected in the chapters which describe the protocols that allow use of these modern approaches. The preparation of this book has required participation of several scientists, all leading experts in their respective fields. Without their enthusiastic participation, this work would not have been possible. Therefore, I thank all authors for their contri- tionsandacceptallcriticismformissingparts,orinformationordetails,forwhichonly I am responsible. I hope these protocols will be useful for young investigators who approach for the first time the complex field of immunology and for those more experienced scientists who look for concise and efficacious descriptions of novel methods.

Provides an easily accessible reference volume for cell-based phenotypic screening, written by leading researchers in the field

Covers a wide range of cell-based assay formats and systems

Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and approaches to data analysis

Comprehensive guide to developing, running, and analyzing cell-based assays



Klappentext

With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls.

Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.



Zusammenfassung
The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches.

Inhalt
Chapter 1: Analysis of Frequency and Phenotype of Antigen-Specific T Cells Angus Stock and Vincenzo Cerundolo Chapter 2: B Cell Helper Assays Sergio Abrignani, Elena Tonti, Giulia Casorati, and Paolo Dellabona Chapter 3: Trans-Kingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing Thu A. Nguyen and Johannes H. Fruehauf Chapter 4: Flow Cytometry and Cell Activation Sonia Gavasso Chapter 5: Investigating T Cells by Polychromatic Flow Cytometry Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza Chapter 6: Generation of Human T Cell Clones Sabrina Mariotti and Roberto Nisini Chapter 7: Limiting Dilution Analysis of Antigen-Specific T cells Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan Chapter 8: T Cell Epitope-Mapping by Cytokine Gene Expression Assay Maurizio Provengano and Giulio C. Spagnoli Chapter 9: Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications Wilco de Jager, Berent Prakken, and Ger T. Rijkers Chapter 10: Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE Mahima Swamy and Wolfgang W.A. Schamel Chapter 11: Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation Paul Zajac

Produktinformationen

Titel: T Cell Protocols
Editor:
EAN: 9781588295873
ISBN: 1588295877
Format: Fester Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 184
Gewicht: 661g
Größe: H266mm x B198mm x T16mm
Jahr: 2008
Auflage: 2nd ed. 2009

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