Suppression and Regulation of Immune Responses

This book highlights recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins, as well as the role of several suppressive molecules in immunoregulation.

Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation. In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins such as B, NK, myeloid, and dendritic cells, as well as the role of several suppressive molecules in immunoregulation. Particular emphasis is placed on the characterization of the molecular mechanisms and the therapeutic applications of regulatory cells and molecules in human diseases. Written as a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Comprehensive and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols serves as a key reference for scientists seeking a way toward greater control over the enormous power of the complex and vital immune system.

Features the most recent advances in the field of immunoregulation with detailed but accessible chapters Contains many step-by-step, laboratory protocols complete with lists of materials and vital notes from the experts Includes chapters on physiological situations where immunoregulation play a central role, as in pregnancy Includes supplementary material: sn.pub/extras

Inhalt
Natural and Induced T CD4+CD25+FOXP3+ Regulatory T Cells.- Polyclonal Expansion of Human CD4+CD25+ Regulatory T Cells.- Methods for In vitro Generation of Human Type 1 Regulatory T (Tr1) Cells.- Ex vivo Generation of Regulatory T-Cells: Characterization and Therapeutic Evaluation in a Model of Chronic Colitis.- Phenotypic and Functional Characterization of CD8+ T Regulatory Cells.- Regulatory CD4- CD8- Double Negative T Cells.- Identifying Regulatory B Cells (B10 Cells) that Produce IL-10 in Mice.- DCs in Immune Tolerance in Steady-State Conditions.- Plasmacytoid Dendritic Cells in Tolerance.- In vitro Generated DC with Tolerogenic Functions: Perspectives for In vivo Cellular Therapy.- Preparation of Mouse Bone Marrow-Derived Dendritic Cells with Immunoregulatory Properties.- Myeloid Derived Suppressor Cells (MDSC): Characterization and Expansion in Models of Endotoxemia and Transplantation.- Human Regulatory Macrophages.- NKT and Tolerance.- Anti-Inflammatory and Immunosuppressive Functions of Mast Cells.- A Mesenchymal Stem Cell Potency Assay.- In Vitro Analyses of the Immunosuppressive Properties of Neural Stem/Progenitor Cells Using Anti-CD3/CD28 Activated T Cells.- Immunoregulatory Properties of Heme Oxygenase-1.- Indoleamine, 2,3-Dioxygenase, and Regulatory Function: Tryptophan Starvation and Beyond.- Regulatory T Cell Enrichment by IFN- Conditioning.- Transforming Growth Factor-Beta: Recent Advances on Its Role in Immune Tolerance.- Induction of Tolerogenic Dendritic Cells by NF-B Blockade and Fc Receptor Modulation.- Fine-Tuning Antitumor Responses through the Control of Galectin-Glycan Interactions: An Overview.- Regulation of Lymphocytes by Nitric Oxide.- Pregnancy: Tolerance and Suppression of Immune Responses.- Organ Transplantation: Modulation of T Cell Activation Pathways Initiated by Cell Surface Receptors to Suppress Graft Rejection.- Immunosuppressive Mechanisms During Viral Infectious Diseases.- Ocular Immune Privilege Sites.-Immunoprivileged Sites: The Testis.