Separation Science and Proteomics

Autorentext
Alexandre Stoyanov, PhD is Assistant Research Professor in the Department of Pathology and Anatomical Sciences at the University of Missouri. He earned his PhD in Chemistry in the Institute of Physical Chemistry at the Russian Academy of Sciences in Moscow. In 2001 Dr. Stoyanov co-authored the Elsevier book, Proteome Revisited: Theory and Practice of all Relevant Electrophoretic Steps. He has been a contributor to two other books and more than 30 journal articles on related topics. His current research focuses on electophoretic and chromatographic separations, spectroscopy, 2D electrophoresis and LC-mass spectrometry.

Klappentext

Separation Science and Proteomics: Current Trends and New Approaches provides readers with the most advanced theories and methodologies on the most important separation methods contributing to current proteomics. Intended to be a practical reference that meets the needs of everyday practitioners, the book also serves as a go-to guide for scientists focused on the theory of separation science, new method development, and new equipment design. Authored by a chemist who has more than 20 years of experience in separation science and proteomics, this book offers readers insights and solutions to the separation mechanisms of a full range of related techniques and complex problems.

Topics covered include the concentration distribution of analyzed protein during IEF separation in natural or IPG gradients and detailed mechanisms of electrophoresis in thermal gradients.

Inhalt

1. Basic Separation Techniques in Protein Fractionation2. Acid-base equilibria and protein behavior in electrically driven separations3. Non-aqueous media4. Electroosmosis5. Detection Methods6. Multi-dimensional maps and multistep separations7. Non-conventional electrophoretic approaches to protein fractionation8. Sample preparation9. Mass Spectrometry10. Proteomics technologies11. Biophysical Methods contributing to Proteomics