This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Third Edition serves as an ideal guide for researchers working toward furthering our understanding of the aging process.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Protocols to Assess the Aging of Human Mesenchymal Stem Cells.- Analyzing Muscle Stem Cell Function Ex Vivo.- Tubular Aggregates as a Marker of Aging in Skeletal Muscle.- Fusion of Human Synovium-Derived Mesenchymal Stem/Stromal Cells with Primary Human Chondrocytes Using the Modified Adherence Method (MAM).- Multiplex PCR for the Rapid Diagnosis of Myeloproliferative Neoplasms.- Ex Vivo Expansion of Cord Blood Hematopoietic Stem and Progenitor Cells.- Evaluation of Anti-Aging Agents Using the D-Galactose-Induced Accelerated Aging Model.- Methods to Detect and Compare Cellular and Mitochondrial Changes in Senescent and Healthy Mesenchymal Stem Cells.- Transfer of Mitochondria from Healthy Stem Cells to Injured Cells in Stroke with Retinal Impairments.- A Protocol for Detecting DNA Methylation Changes at CpG Sites of Stemness-Related Genes in Aging Stem Cells.- Robust Expansion of Hematopoietic Stem Cells Ex Vivo Using Small Molecule Cocktails.- A Genome-Wide CRISPR/Cas9 Screen Identifies Regulatory Genes for Stem Cell Aging.- Immunocompetent Brain Organoids with Microglia Allow Advanced Aging Research.- Quality Control in Human Adipose-Derived Stromal/Stem Cells and Tissue Engineering Fat Models for Aging Studies.- Mesenchymal Stromal Cell (MSC) Isolation and Induction of Acute and Replicative Senescence.- Delivery of Stem Cell Rejuvenating Compounds via Subcutaneous Osmotic Pumps.- Isolation Procedure for Rat Pancreatic Ductal Cells.- Polychromatic Flow Cytometry to Identify Rare Aged Hematopoietic Stem Cell Subpopulations.- Isolation of Human Hematopoietic Stem Cells from an Apheresis Sample.- Quantifying Muscle Regeneration: Activated Muscle Satellite Cells and New Regenerated Myofibers in Chronic and Acute Degeneration Models.- Identification of De Novo Dividing Stem Cells.- Rat Ductal Cell-Derived Differentiation into Islet-Like Cells.