Autorentext

STEFAN LINDER is Professor of Cellular Microbiology at University Medical Center Hamburg-Eppendorf (UKE), Germany. He is a member of the Life Science Committee at the Royal Microscopical Society, and he serves on the editorial boards of European Journal of Cell Biology (EJCB) and Frontiers in Immunology. CLAIRE M. WELLS is Professor of Cancer Cell Biology at Kings College London, UK. She is a member of the British Society for Cell Biology and a member of the Life Science Committee at the Royal Microscopical Society. She is Editor in Chief of Cell Adhesion and Migration.

Klappentext

COMPREHENSIVE REFERENCE EXPLORING MICROSCOPY TECHNIQUES TO SUPPORT THE STUDY OF LIVING SYSTEMS WITH A UNIQUE MULTISCALE FOCUS Live Biological Imaging Across Scales presents an overview of the technologies and experimental strategies available for live imaging across scales. Each chapter highlights a different technique, working upwards from small to large scale. Readers will find critical evaluation of these techniques and perspectives on future developments in this evolving field, enabling them to effectively identify techniques that may benefit their research activities. Written by a team of leading experts and part of the Wiley-Royal Microscopical Society series, Live Biological Imaging Across Scales includes information on:

• Single cells, covering single molecule tracking/FRET, actin, microtubules, intermediate filaments, septins, vesicles, phagosomes, and confined environments
• Multiple cells, covering collective migration, endothelial monolayers, flow platelets, and flow, wound healing, monocyte transmigration, cancer spheroids/tumor models, and organoids
• Organisms, covering Dictyostelium, C. elegans, Drosophila, and mouse-tumors
• Imaging of whole cells individually and collectively in both 2D and 3D platforms Live Biological Imaging Across Scales is an essential reference for microscopists and experimental biologists, particularly those interested and/or working in the expanding field of live imaging in biology, as well as those working in the commercial microscopy industry supply and development.

Inhalt

List of Contributors xi

Preface xiii

1 Super-Resolution Imaging 1

2 Peering into the Septin Cytoskeleton -New Insights Unlocked by Microscopy Advances 23

3 Imaging Phagocytosis: From Cells to Molecules 43

4 Imaging Wound Healing Across Scales 65

5 Leukocyte and Cancer Adhesion Live-Cell Imaging 81

6 Bridging the Gap Between In Vitro and In Vivo Studies with Live-Cell Imaging of Organoids 97

7 Tiny Organisms, Big Advantages: Exploring Membrane Damage Responses Using Dictyostelium discoideum in High-Throughput Single-Cell Analyses 123

8 Live Imaging in Caenorhabditis elegans Neurons 141

9 It's Just a Scratch -Lessons from Epidermal Wound Closure in Drosophila 171

10 Intravital Imaging of Tumors 191

Index 211